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QuicKey Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit

Uniprot : P80188
  • Cat.No.:E-TSEL-H0003

  • Reactivity: Human

To Purchase E-TSEL-H0003

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $390
Qty:

Product Details

QuicKey Series

Get more sensitive and precise results with saving at least 1h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 2.5h
Detection range 0.13-8 ng/mL
Sensitivity 0.06 ng/mL
Sample type &Sample volume serum, plasma, urine; 50μL
Specificity This kit recognizes Human NGAL in samples. No significant or interference between Human NGAL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human NGAL concentrations in serum, plasma, urine.Please consult technical support for the applicability if other biological fluids need to be tested.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGAL. Samples (or Standards) and biotinylated detection antibody specific for Human NGAL are added to the micro ELISA plate wells. Human NGAL would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human NGAL. You can calculate the concentration of Human NGAL in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.
ItemSpecificationsStorage conditions after test
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
2-8℃, 1 month
Reference Standard 96T: 2 vials
48T: 1 vial
Discard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃
Biotinylated Detection Ab Working Solution 1 vial, 6 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
2-8℃ (Protect from light)
Substrate Reagent 1 vial, 10 mL
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Sample Values

sandwich-Ab-ELISA-Elabscience

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human NGAL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human NGAL were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.37 0.90 3.57 0.35 0.81 3.73
Standard deviation 0.02 0.04 0.16 0.02 0.04 0.17
CV (%) 5.41 4.44 4.48 5.71 4.94 4.56

Recovery

The recovery of Human NGAL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 94
EDTA plasma(n=8) 101-110 104
Urine(n=8) 87-98 90

Linearity

Samples were spiked with high concentrations of Human NGAL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

sandwich-Ab-ELISA-Elabscience

Target Information

Background

Neutrophil gelatinase associated lipocalin (NGAL) also known as Lipocalin 2 or Lcn2 is a 25 kDa, is a member of the lipocalin superfamily and a pleiotropic mediator of various inflammatory processes [1].NGAL, identified originally as a protein associated with matrix metalloproteinase 9 (MMP-9) of human neutrophils. Lipocalins are extracellular proteins which share a common tertiary structure that forms a barrel-like hydrophobic ligand binding site. When bound to MMP-9, NGAL protects it from proteolytic degradation sustaining the proteolytic activity of MMP-9. Neutrophil gelatinase-associated lipocalin (NGAL), is released by various cell types and is an attractive biomarker of inflammation, ischemia, infection, and kidney damage. Both intestinal and metabolic inflammation, as observed in obesity and related disorders, are associated with increased NGAL synthesis [2]. While NGAL in the intestinal tract regulates the composition of the gut microbiota and shows anti-inflammatory activities, it also exhibits proinflammatory activities in other experimental settings. Plasma NGAL is a novel marker of kidney function, which correlates to duration of end-stage renal failure (ESRD) and serum creatinine in uremic patients awaiting kidney transplantation. Plasma NGAL is associated with homocysteine in transplanted patients.
1. Kjeldsen L, Cowland J B, Borregaard N. Human neutrophil gelatinase-associated lipocalin and homologous proteins in rat and mouse.[J]. Biochim Biophys Acta, 2000, 1482(1):272-283.
2. Tilg, H., & Moschen, A. R. Adipocytokines: mediators linking adipose tissue, inflammation and immunity. Nature Reviews Immunology, 2006, 6(10), 772.
Database LinksSwissProt: P80188
Entrez Gene: 3934
SynonymsLCN2, Lipocalin 2, Oncogene 24p3, MSFI
Research AreaImmunology,Cancer,Signaling transduction

Assay Procedures

step01

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C

step02

2. Aspirate and wash the plate for 3 times

step03

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

step04

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

step05

5. Add 50μL Stop Solution

step06

6. Read the plate at 450nm immediately. Calculation of the results

Citations

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