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QuicKey Pro Human CEA(Carcinoembryonic Antigen) ELISA Kit

Citations(7)Uniprot : P06731

Cat.No.:E-OSEL-H0016
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	0.78-50 ng/mL
Sensitivity	0.47 ng/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes CEA in samples. No significant cross-reactivity or interference between CEA and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of CEA concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CEA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CEA are added to the micro ELISA plate wells. Human CEA in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to CEA. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level CEA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level CEA were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.44	7.17	18.89	2.29	6.53	19.86
Standard deviation	0.13	0.40	0.60	0.13	0.26	0.66
CV (%)	5.16	5.51	3.16	5.79	4.01	3.32

Recovery

The recovery of CEA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-107	100
EDTA plasma(n=8)	87-98	92

Linearity

Samples were spiked with high concentrations of Human CEA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P06731
Synonyms	CEA，Carcinoembryonic Antigen
Research Area	Cancer, Immunology, Stem cells, Tags and Cell Markers

Assay Procedures

Citations

BIOSENSORS & BIOELECTRONICS (2022) IF: 10.618
Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis

DOI: 10.1016/j.bios.2022.114487

PMID: 35780540
Sample: exosomes
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020) IF: 9.4120
Development of a serum miRNA panel for detection of early stage non-small cell lung cancer

DOI: 10.1073/pnas.2006212117

PMID: 32943537
Sample: Serum
TALANTA (2021) IF: 6.057
Fluorescence-infrared absorption dual-mode nanoprobes based on carbon dots@SiO2 nanorods for ultrasensitive and reliable detection of carcinoembryonic antigen

DOI: 10.1016/j.talanta.2021.122342

Sample: Serum
European Review for Medical and Pharmacological Sciences (2020) IF: 3.024
Hsa_circ_0124055 and hsa_circ_0101622 regulate proliferation and apoptosis in thyroid cancer and serve as prognostic and diagnostic indicators

DOI: 10.26355/eurrev_202004_21016

PMID: 32373972
Sample: Serum,Tissue homogenate
ANALYST (2020) IF: 3.978
In situ amplified QCM immunoassay for carcinoembryonic antigen with colorectal cancer using horseradish peroxidase nanospheres and enzymatic biocatalytic precipitation

DOI: 10.1039/D0AN01399D

Sample: serum
Analytical Methods (2018) IF: 2.073
A potentiometric aptasensor for carcinoembryonic antigen (CEA) on graphene oxide nanosheets using catalytic recycling of DNase I with signal amplification

DOI: 10.1039/C8AY02113A

Sample: serum
Cancer Biomarkers (2016) IF: 1.736
Macrophage mannose receptor 1 and S100A9 were identified as serum diagnostic biomarkers for colorectal cancer through a label-free quantitative proteomic analysis

DOI: 10.3233/CBM-150560

Sample: serum

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