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QuicKey Pro Human Cortisol ELISA Kit

Cat.No.:E-OSEL-H0006
Reactivity: Human

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	6.25-400 ng/mL
Sensitivity	2.87 ng/mL
Sample type &Sample volume	serum, plasma, urine, saliva; 50μL
Specificity	This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Cortisol concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Cortisol are added to the micro ELISA plate wells. Human Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Cortisol were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Cortisol were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	18.87	58.54	147.36	18.78	63.49	141.75
Standard deviation	1.28	3.40	5.56	0.97	3.58	5.07
CV (%)	6.79	5.80	3.77	5.19	5.64	3.58

Recovery

The recovery of Cortisol spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	84-96	90
EDTA plasma(n=8)	90-103	96
Urine(n=8)	91-109	99

Linearity

Samples were spiked with high concentrations of Human Cortisol and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	11β,17α,21-Trihydroxypregn-4-ene-3,20-dione
Research Area	Cell biology, Metabolism

Assay Procedures

Citations

ENVIRONMENTAL POLLUTION (2022) IF: 9.988
Neuroendocrine stress hormones associated with short-term exposure to nitrogen dioxide and fine particulate matter in individuals with and without chronic obstructive pulmonary disease: A panel study in Beijing, China

DOI: 10.1016/j.envpol.2022.119822

PMID: 35870527
Sample: serum
BRAIN BEHAVIOR AND IMMUNITY (2021) IF: 7.217
Bifidobacterium breve CCFM1025 attenuates major depression disorder via regulating gut microbiome and tryptophan metabolism: A randomized clinical trial

DOI: 10.1016/j.bbi.2021.11.023

PMID: 34875345
Sample: serum
BIOSENSORS & BIOELECTRONICS (2016) IF: 7.476
Investigate electrochemical immunosensor of cortisol based on gold nanoparticles/magnetic functionalized reduced graphene oxide

DOI: 10.1016/j.bios.2016.07.047

Sample: Others
Journal of the International Society of Sports Nutrition (2020) IF: 5.068
Caffeine supplementation induces higher IL-6 and IL-10 plasma levels in response to a treadmill exercise test

DOI: 10.1186/s12970-020-00375-4

PMID: 32907591
Sample: Plasma
Frontiers in Physiology (2021) IF: 4.566
Human Physiological Responses to a Single Deep Helium-Oxygen Diving

DOI: 10.3389/fphys.2021.735986

Sample: saliva
Annals of Translational Medicine (2022) IF: 3.616
Mechanism of the Huangguoshu waterfall forest environment's promotive effect on human health in Guizhou, China.

DOI: 10.21037/atm-22-3787

Sample: serum
MEDICINE (2018) IF: 2.028
An investigation into psychological stress and its determinants in Xinjiang desert oil workers

DOI: 10.1097/MD.0000000000010323

Sample: serum
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
The Effect of Copaiba Oil Odor on Anxiety Relief in Adults under Mental Workload: A Randomized Controlled Trial

DOI: 10.1155/2022/3874745

PMID: 35449818
Sample: saliva
Drug Design Development and Therapy (2017) IF: 2.822
Rutin protects against neuronal damage in vitro and ameliorates doxorubicin-induced memory deficits in vivo in Wistar rats

DOI: 10.2147/DDDT.S103511

Sample: Serum
JOURNAL OF OBSTETRICS AND GYNAECOLOGY (2018) IF: 0.545
Stress response to ovariohysterectomy in rabbits: role of anaesthesia and surgery

DOI: 10.1080/01443615.2017.1400523

Sample: Serum,Plasma

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