Current Position:Home>>Hot>>QuicKey Pro ELISA>> QuicKey Pro Rat IgG(Immunoglobulin G) ELISA Kit

QuicKey Pro Rat IgG(Immunoglobulin G) ELISA Kit

Cat.No.:E-OSEL-R0005
Reactivity: Rat

Filter:

To Purchase E-OSEL-R0005

Size:

96T
48T
24T
96T*5
96T*10

Price: $520

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	7.81-500 ng/mL
Sensitivity	2.98 ng/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes IgG in samples. No significant cross-reactivity or interference between IgG and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of IgG concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IgG are added to the micro ELISA plate wells. Rat IgG in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IgG. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IgG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IgG were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	23.38	68.80	230.89	21.47	74.91	214.43
Standard deviation	1.42	3.51	7.37	1.34	3.26	6.65
CV (%)	6.08	5.10	3.19	6.26	4.35	3.10

Recovery

The recovery of IgG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-102	94
EDTA plasma(n=8)	85-97	90

Linearity

Samples were spiked with high concentrations of Rat IgG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Immunoglobulin G,IgG
Research Area	Immunology

Assay Procedures

Citations

Frontiers in Bioengineering and Biotechnology (2022) IF: 6.064
Safety and biodistribution of exosomes derived from human induced pluripotent stem cells.

DOI: 10.3389/fbioe.2022.949724

Sample: Blood
Scientific Reports (2016) IF: 5.228
Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy

DOI: 10.1038/srep31771

Sample: Urine
FASEB JOURNAL (2018) IF: 5.595
A novel immunodeficient rat model supports human lung cancer xenografts

DOI: 10.1096/fj.201800102RR

Sample: Serum
Dose-Response (2022) IF: 2.623
Pharmacological Role of Capparis decidua (Forssk.) Edgew in Preventing Cyclophosphamide-induced Myelosuppression and Modulating Innate and Adaptive Immune Response:

DOI: 10.1177/15593258221123672

Sample: serum
Journal of Zhejiang University-SCIENCE B (2019) IF: 1.879
Improvement in post-partum uterine involution in rats treated with Apios americana

DOI: 10.1631/jzus.B1800475

Sample: serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha