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Cat.No.:E-EL-R3002
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 7.81-500 pg/mL |
Sensitivity | 4.69 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Rat hs-CRP in samples. No significant cross-reactivity or interference between Rat hs-CRP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Rat hs-CRP concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat hs-CRP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat hs-CRP were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 20.13 | 56.75 | 235.50 | 20.39 | 60.86 | 222.49 |
Standard deviation | 1.20 | 2.81 | 12.29 | 1.07 | 2.87 | 9.52 |
CV (%) | 5.96 | 4.95 | 5.22 | 5.25 | 4.72 | 4.28 |
The recovery of Rat hs-CRP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 89-97 | 93 |
EDTA plasma (n=8) | 97-109 | 105 |
Cell culture media (n=8) | 90-101 | 98 |
Samples were spiked with high concentrations of Rat hs-CRP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
P48199
|
Synonyms | PTX1, Pentraxin 1 |
Research Area | Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Immunology |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
DOI: 10.3390/jcm10143143 |
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PMID: 34300308 |
Sample: plasma |
DOI: 10.7717/peerj.13990 |
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PMID: 36213511 |
Sample: serum |
DOI: 10.1155/2020/1936406 |
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PMID: 32117470 |
Sample: Plasma,Tissue homogenate |
Sample: plasma |
Sample: serum |
B**aSubmitted [ Oct 26 2023 ]
Asked: Can I use heparin plasma to analyse rat hs-CRP using this kit (E-EL-R3002)?
Reply
adminSubmitted [ Oct 26 2023 ]
Answered: Heparin sodium anticoagulant tube is not suitable for thrombin related detection, it has strong anticoagulant ability and is not easy to hemolysis. CRP is an important promoter of the exogenous clotting pathway, so the use of heparin anticoagulants is not recommended.
adminSubmitted [ Oct 26 2023 ]
Answered:
B**aSubmitted [ Oct 26 2023 ]
Asked: Is it possible to use K3EDTA plasma sample to analyse CRP plasma using this kit (Cat.No.:E-EL-R3002)?
Reply
adminSubmitted [ Oct 26 2023 ]
Answered: In general, EDTA-2K has stronger stability and better safety than -3K, it is more stable under physiological conditions and has less impact on blood cells. However, the differences between them are subtle. If you don't do a very detailed analysis or reaction, the difference is not obvious.