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Rat I-PTH(Intact Parathormone) ELISA Kit

Cat.No.:E-EL-R0535
Reactivity: Rat

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat I-PTH in samples. No significant cross-reactivity or interference between Rat I-PTH and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat I-PTH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat I-PTH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat I-PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat I-PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat I-PTH. You can calculate the concentration of Rat I-PTH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat I-PTH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat I-PTH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	49.45	154.88	373.68	45.17	166.01	402.93
Standard deviation	2.60	6.91	19.51	3.04	7.29	13.94
CV (%)	5.26	4.46	5.22	6.73	4.39	3.46

Recovery

The recovery of Rat I-PTH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-102	95
EDTA plasma (n=8)	92-107	98
Cell culture media (n=8)	94-109	101

Linearity

Samples were spiked with high concentrations of Rat I-PTH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

LIFE SCIENCES (2022) IF: 6.78
The Cannabinoid-2 receptor agonist, 1-phenylisatin, protects against cisplatin-induced nephrotoxicity in mice

DOI: 10.1016/j.lfs.2022.120928

PMID: 36058263
Sample: kidney tissue
Stem Cell Research & Therapy (2020) IF: 6.832
Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model

DOI: 10.1186/s13287-020-01733-4

PMID: 32456711
Sample: Plasma
Food & Function (2022) IF: 5.396
Diosmin, a citrus fruit-derived phlebotonic bioflavonoid protects rats from chronic kidney disease-induced loss of bone mass and strength without deteriorating the renal function

DOI: 10.1039/D1FO03867B

PMID: 35119062
Sample: serum
BONE (2020) IF: 4.147
Calcium repletion to rats with calcipenic rickets fails to recover bone quality: A calcipenic “memory”

DOI: 10.1016/j.bone.2020.115562

Sample: serum
Frontiers in Pharmacology (2020) IF: 4.225
Bazedoxifene Attenuates Abdominal Aortic Aneurysm Formation via Downregulation of Interleukin-6/Glycoprotein 130/Signal Transducer and Activator of Transcription 3 Signaling Pathway in Apolipoprotein E–Knockout Mice

DOI: 10.3389/fphar.2020.00392

Sample: Cell culture medium
Frontiers in Physiology (2023) IF: 4.755
Relative comparison of chronic kidney disease-mineral and bone disorder rat models

DOI: 10.3389/fphys.2023.1083725

Sample: Serum
Oxidative Medicine and Cellular Longevity (2019) IF: 4.868
Beneficial Effects of Total Phenylethanoid Glycoside Fraction Isolated from Cistanche deserticola on Bone Microstructure in Ovariectomized Rats

DOI: 10.1155/2019/2370862

PMID: 31346358
Sample: Serum,Urine
BIOMETALS (2022) IF: 3.378
Iron overload impairs renal function and is associated with vascular calcification in rat aorta

DOI: 10.1007/s10534-022-00449-7

PMID: 36178540
Sample: serum
ENDOCRINE (2022) IF: 3.925
Roxadustat improves renal osteodystrophy by dual regulation of bone remodeling

DOI: 10.1007/s12020-022-03199-1

PMID: 36184719
Sample: serum
Annals of Translational Medicine (2021) IF: 3.932
Conjugated linoleic acid prompts bone formation in ovariectomized osteoporotic rats and weakens osteoclast formation after treatment with ultraviolet B

DOI: 10.21037/atm-21-934

PMID: 33850900
Sample: Serum
Experimental and Therapeutic Medicine (2018) IF: 1.41
Effect of parathyroid hormone on cardiac function in rats with cardiomyopathy

DOI: 10.3892/etm.2018.6528

PMID: 30214507
Sample: Serum

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