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Cat.No.:E-EL-R0566
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Rat IL-17A in samples. No significant cross-reactivity or interference between Rat IL-17A and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Rat IL-17A concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-17A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-17A were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 55.20 | 149.70 | 486.20 | 50.50 | 153.30 | 468.50 |
Standard deviation | 3.40 | 7.90 | 18.00 | 2.60 | 8.70 | 21.60 |
CV (%) | 6.16 | 5.28 | 3.70 | 5.15 | 5.68 | 4.61 |
The recovery of Rat IL-17A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 95-110 | 101 |
EDTA plasma (n=8) | 97-111 | 102 |
Cell culture media (n=8) | 93-104 | 99 |
Samples were spiked with high concentrations of Rat IL-17A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
Q61453
|
Synonyms | CTLA8, CTLA-8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17, IL17A, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8 |
Research Area | Cancer, Immunology |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
Sample: Plasma |
Sample: serum |
Sample: skin tissue |
Sample: serum |
DOI: 10.3390/ph15111412 |
|
Sample: intestinal tissue |
PMID: 34911003 |
Sample: serum |
DOI: 10.1002/tox.23456 |
|
PMID: 35014750 |
Sample: cardiac tissue |
DOI: 10.1002/tox.23324 |
|
Sample: serum,liver |
PMID: 34764759 |
Sample: brain |
Sample: Serum |
PMID: 34737112 |
Sample: serum |
DOI: 10.1111/jgh.15987 |
|
Sample: blood,colon |
Sample: serum |
DOI: 10.1002/ptr.5775 |
|
Sample: Cell culture supernatant |
Sample: fibroblast-like synoviocytes (FLS) |
PMID: 34719315 |
Sample: serum |
PMID: 34911449 |
Sample: hippocampus tissue |
Sample: Serum |
DOI: 10.1155/2022/7812099 |
|
PMID: 36246554 |
Sample: serum,kidney tissues |
Sample: serum |
Sample: fibroblast-like synoviocytes (FLSs) |
DOI: 10.1002/brb3.1478 |
|
PMID: 31742934 |
Sample: Serum,Tissue homogenate |
PMID: 33149866 |
Sample: Tissue homogenate |
PMID: 32670559 |
Sample: Tissue homogenate |
PMID: 35796694 |
Sample: Liver tissue |
Sample: Cell lysate |
Sample: Cell culture supernatant |
Sample: Tissue homogenate |
DOI: 10.1002/jcb.28243 |
|
PMID: 30520122 |
Sample: Serum,Bronchoalveolar Lavage Fluid |