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Rat IL-18(Interleukin 18) ELISA Kit

Citations(72)Uniprot : P97636

  • Cat.No.:E-EL-R0567

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IL-18 in samples. No significant cross-reactivity or interference between Rat IL-18 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IL-18 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-18. You can calculate the concentration of Rat IL-18 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-18 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-18 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 52.00 118.30 412.00 56.70 128.40 387.30
Standard deviation 3.60 4.90 21.00 3.10 6.50 12.80
CV (%) 6.92 4.14 5.10 5.47 5.06 3.30

Recovery

The recovery of Rat IL-18 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-100 91
EDTA plasma (n=8) 95-108 101
Cell culture media (n=8) 88-102 93

Linearity

Samples were spiked with high concentrations of Rat IL-18 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P97636
SynonymsIL18, IGIF, IL-1g, IL-1F4, Interferon-gamma-inducing factor
Research AreaCancer, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Frontiers in Immunology (2022) IF: 8.786
    Dynamic Monitoring of Immunoinflammatory Response Identifies Immunoswitching Characteristics of Severe Acute Pancreatitis in Rats

    DOI: 10.3389/fimmu.2022.876168
    Sample: serum
  2. Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
    Exosomal miR-27b-3p Derived from Hypoxic Cardiac Microvascular Endothelial Cells Alleviates Rat Myocardial Ischemia/Reperfusion Injury through Inhibiting Oxidative Stress-Induced Pyroptosis via Foxo1/GSDMD Signaling

    DOI: 10.1155/2022/8215842

    PMID: 35847592

    		 Sample: Serum,H9C2 cell
  3. Cells (2022) IF: 7.666
    Melatonin Attenuates Spinal Cord Injury in Mice by Activating the Nrf2/ARE Signaling Pathway to Inhibit the NLRP3 Inflammasome

    DOI: 10.3390/cells11182809

    PMID: 36139384

    		 Sample: PC12 cell
  4. Cells (2022) IF: 7.666
    Hypoxia-Induced HIF-1α Expression Promotes Neurogenic Bladder Fibrosis via EMT and Pyroptosis

    DOI: 10.3390/cells11233836
    Sample: serum
  5. BIOCHEMICAL PHARMACOLOGY (2023) IF: 6.1
    Modulation of p38 MAPK and Nrf2/HO-1/NLRP3 inflammasome signaling and pyroptosis outline the anti-neuroinflammatory and remyelinating characters of Clemastine in EAE rat model

    DOI: 10.1016/j.bcp.2023.115435
    Sample: brain tissue
  6. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021) IF: 6.291
    Bone marrow-derived mesenchymal stem cells attenuate silica-induced pulmonary fibrosis by inhibiting apoptosis and pyroptosis but not autophagy in rats

    DOI: 10.1016/j.ecoenv.2021.112181

    PMID: 33848736

    		 Sample: Cell sample,Tissue Slice
  7. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    Pharmacological evaluation of continentalic acid for antidiabetic potential

    DOI: 10.1016/j.biopha.2021.111411

    PMID: 33711550

    		 Sample: Whole Blood
  8. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Pentoxifylline and berberine mitigate diclofenac-induced acute nephrotoxicity in male rats via modulation of inflammation and oxidative stress

    DOI: 10.1016/j.biopha.2022.113225

    PMID: 35671584

    		 Sample: serum
  9. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    MCC950 attenuates doxorubicin-induced myocardial injury in vivo and in vitro by inhibiting NLRP3-mediated pyroptosis

    DOI: 10.1016/j.biopha.2021.112133

    PMID: 34474337

    		 Sample: H9c2 cell
  10. Oxidative Medicine and Cellular Longevity (2016) IF: 6.543
    Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes

    DOI: 10.1155/2016/2386068
    Sample: Tissue homogenate
  11. LIFE SCIENCES (2022) IF: 6.78
    Astragaloside IV ameliorates sepsis-induced myocardial dysfunction by regulating NOX4/JNK/BAX pathway

    DOI: 10.1016/j.lfs.2022.121123

    PMID: 36302499

    		 Sample: myocardium,serum
  12. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    Ellagic Acid Protects Dopamine Neurons via Inhibition of NLRP3 Inflammasome Activation in Microglia

    DOI: 10.1155/2020/2963540

    PMID: 33294118

    		 Sample: Cell culture medium
  13. JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
    Optimal timing of free total rhubarb anthraquinones on immune regulation in rats with severe acute pancreatitis

    DOI: 10.1016/j.jep.2023.116266
    Sample: blood,Small intestine tissue
  14. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Exploring the anti-inflammatory potential of Colocasia esculenta root extract in in-vitro and in-vivo models of inflammation

    DOI: 10.1016/j.jep.2022.116021
    Sample: serum
  15. Frontiers in Molecular Biosciences (2021) IF: 5.246
    Folic Acid Alleviates High Glucose and Fat-Induced Pyroptosis via Inhibition of the Hippo Signal Pathway on H9C2 Cells

    DOI: 10.3389/fmolb.2021.698698

    PMID: 34692767

    		 Sample: H9C2 cell
  16. Frontiers in Cellular and Infection Microbiology (2022) IF: 5.293
    Intestinal Epithelial Cell Exosome Launches IL-1β-Mediated Neuron Injury in Sepsis-Associated Encephalopathy

    DOI: 10.3389/fcimb.2021.783049

    PMID: 35111693

    		 Sample: serum,hippocampal tissue
  17. PHYTOMEDICINE (2022) IF: 5.34
    Piperazine ferulate attenuates gentamicin-induced acute kidney injury via the NF-κB/NLRP3 pathway

    DOI: 10.1016/j.phymed.2022.154021

    PMID: 35286937

    		 Sample: serum
  18. MOLECULAR NEUROBIOLOGY (2022) IF: 5.59
    Targeting the TLR4/NF-κΒ Axis and NLRP1/3 Inflammasomes by Rosuvastatin: A Role in Impeding Ovariectomy-Induced Cognitive Decline Neuropathology in Rats

    DOI: 10.1007/s12035-022-02852-0

    PMID: 35578102

    		 Sample: hippocampus
  19. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    Protective effect of fatty acid amide hydrolase inhibitor URB597 and monoacylglycerol lipase inhibitor KML29 on renal ischemia–reperfusion injury via toll-like receptor 4/nuclear factor-kappa B pathway

    DOI: 10.1016/j.intimp.2022.109586
    Sample: serum
  20. Frontiers in Aging Neuroscience (2021) IF: 5.75
    Enriched Environment Attenuates Pyroptosis to Improve Functional Recovery After Cerebral Ischemia/Reperfusion Injury

    DOI: 10.3389/fnagi.2021.717644

    PMID: 34646128

    		 Sample: penumbra tissue
  21. Journal of Neuroinflammation (2020) IF: 5.793
    Sodium para-aminosalicylic acid inhibits manganese-induced NLRP3 inflammasome-dependent pyroptosis by inhibiting NF-κB pathway activation and oxidative stress

    DOI: 10.1186/s12974-020-02018-6

    PMID: 33203418

    		 Sample: Tissue homogenate
  22. Frontiers in Pharmacology (2021) IF: 5.811
    Sinapine Thiocyanate Ameliorates Vascular Endothelial Dysfunction in Hypertension by Inhibiting Activation of the NLRP3 Inflammasome

    DOI: 10.3389/fphar.2020.620159

    PMID: 33633569

    		 Sample: Serum
  23. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2021) IF: 4.013
    Nicotinamide mononucleotide attenuates doxorubicin-induced cardiotoxicity by reducing oxidative stress, inflammation and apoptosis in rats

    DOI: 10.1016/j.abb.2021.109050

    PMID: 34610336

    		 Sample: plasma, heart
  24. ENVIRONMENTAL TOXICOLOGY (2021) IF: 4.119
    Effects of 2,4-dichlorophenoxyacetic acid on the expression of NLRP3 inflammasome and autophagy-related proteins as well as the protective effect of Lycium barbarum polysaccharide in neonatal rats

    DOI: 10.1002/tox.23358

    PMID: 34464015

    		 Sample: serum
  25. ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.119
    2,4-dichlorophenoxyacetic acid induces ROS activation in NLRP3 inflammatory body-induced autophagy disorder in microglia and the protective effect of Lycium barbarum polysaccharide

    DOI: 10.1002/tox.23471

    PMID: 35099110

    		 Sample: serum
  26. LABORATORY INVESTIGATION (2018) IF: 4.263
    The role of Ca 2+ in acid-sensing ion channel 1a-mediated chondrocyte pyroptosis in rat adjuvant arthritis

    DOI: 10.1038/s41374-018-0135-3

    PMID: 30487596

    		 Sample: Plasma
  27. CELL BIOLOGY INTERNATIONAL (2023) IF: 4.473
    TFEB attenuates hyperglycemia-induced retinal capillary endothelial cells injury via autophagy regulation

    DOI: 10.1002/cbin.12002
    Sample: serum
  28. INFLAMMOPHARMACOLOGY (2021) IF: 4.473
    Exploring the role of Aquaporins (AQPs) in LPS induced systemic inflammation and the ameliorative effect of Garcinia in male Wistar rat

    DOI: 10.1007/s10787-021-00832-9

    PMID: 34106384

    		 Sample: serum
  29. TOXICOLOGY (2022) IF: 4.571
    MicroRNA-223-3p promotes pyroptosis of cardiomyocyte and release of inflammasome factors via downregulating the expression level of SPI1 (PU.1)

    DOI: 10.1016/j.tox.2022.153252

    PMID: 35792203

    		 Sample: H9C2 cell
  30. Frontiers in Immunology (2019) IF: 4.716
    Naringenin Produces Neuroprotection Against LPS-Induced Dopamine Neurotoxicity via the Inhibition of Microglial NLRP3 Inflammasome Activation

    DOI: 10.3389/fimmu.2019.00936
    Sample: BV-2 cells
  31. Aging-US (2020) IF: 4.831
    Salidroside ameliorates Parkinson's disease by inhibiting NLRP3-dependent pyroptosis

    DOI: 10.18632/aging.103215

    PMID: 32432571

    		 Sample: Cell culture supernatant,Tissue homogenate
  32. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Suppression of NLRP3 inflammasome by Platycodin D via the TLR4/MyD88/NF-κB pathway contributes to attenuation of lipopolysaccharide induced acute lung injury in rats

    DOI: 10.1016/j.intimp.2021.107621

    PMID: 33872850

    		 Sample: Bronchoalveolar Lavage Fluid
  33. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Pinocembrin inhibited cardiomyocyte pyroptosis against doxorubicin-induced cardiac dysfunction via regulating Nrf2/Sirt3 signaling pathway

    DOI: 10.1016/j.intimp.2021.107533

    PMID: 33752080

    		 Sample: Serum,Cell culture supernatant
  34. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Fisetin attenuates periodontitis through FGFR1/TLR4/NLRP3 inflammasome pathway

    DOI: 10.1016/j.intimp.2021.107505

    PMID: 33725636

    		 Sample: serum,Cell culture supernatant(THP-1 cells)
  35. Oxidative Medicine and Cellular Longevity (2018) IF: 4.936
    Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Diaphragm Dysfunction in Rats by Reducing Apoptosis and Inflammation through ROS/MAPK and TLR4/NF-κB Signaling Pathways

    DOI: 10.1155/2018/9647809
    Sample: Cells
  36. Journal of Diabetes Research (2019) IF: 3.04
    Lipopolysaccharide (LPS) Aggravates High Glucose- and Hypoxia/Reoxygenation-Induced Injury through Activating ROS-Dependent NLRP3 Inflammasome-Mediated Pyroptosis in H9C2 Cardiomyocytes

    DOI: 10.1155/2019/8151836
    Sample: Cell culture supernatant
  37. RSC Advances (2019) IF: 3.049
    Morroniside alleviates coxsackievirus B3-induced myocardial damage apoptosis via restraining NLRP3 inflammasome activation

    DOI: 10.1039/C8RA08662A
    Sample: Tissue homogenate
  38. ENVIRONMENTAL TOXICOLOGY (2020) IF: 3.118
    Methoxytetrahydro-2H-pyran-2-yl)methyl benzoate inhibits spinal cord injury in the rat model via PPAR-γ/PI3K/p-Akt activation

    DOI: 10.1002/tox.22902
    Sample: spinal cord tissue
  39. IMMUNOBIOLOGY (2023) IF: 3.152
    STING mediates SU5416/hypoxia-induced pulmonary arterial hypertension in rats by regulating macrophage NLRP3 inflammasome activation

    DOI: 10.1016/j.imbio.2023.152345
    Sample: lung tissues,Bone marrow-derived macrophage (BMDMs
  40. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2023) IF: 3.195
    Pharmacological evaluation of carvacrol anti-migraine potential

    DOI: 10.1007/s00210-023-02412-w
    Sample: brain
  41. NEUROSCIENCE LETTERS (2022) IF: 3.197
    Anti-depressant effects of acupuncture: The insights from NLRP3 mediated pyroptosis and inflammation

    DOI: 10.1016/j.neulet.2022.136787

    PMID: 35820551

    		 Sample: serum
  42. Annals of Translational Medicine (2020) IF: 3.297
    Palmitic acid stimulates NLRP3 inflammasome activation through TLR4-NF-κB signal pathway in hepatic stellate cells

    DOI: 10.21037/atm.2020.02.21

    PMID: 32309315

    		 Sample: Serum,Cell culture supernatant,Tissue homogenate
  43. Molecular Medicine Reports (2022) IF: 3.423
    Adipose‑derived mesenchymal stem cell‑derived HCAR1 regulates immune response in the attenuation of sepsis

    DOI: 10.3892/mmr.2022.12795

    PMID: 35856408

    		 Sample: blood
  44. Journal of Personalized Medicine (2023) IF: 3.508
    Effect of Hydrogen on AM Pyroptosis Induced by Severe Burns in Rats

    DOI: 10.3390/jpm13030377
    Sample: lung tissues
  45. BRAIN RESEARCH BULLETIN (2023) IF: 3.715
    Acetaldehyde dehydrogenase 2 activation attenuates sepsis-induced brain injury through NLRP3 inflammasome regulation

    DOI: 10.1016/j.brainresbull.2023.01.012
    Sample: serum
  46. BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
    Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma

    DOI: 10.1016/j.biopha.2019.109188

    PMID: 31315072

    		 Sample: Serum,Tissue homogenate
  47. REPRODUCTION (2021) IF: 3.906
    Melatonin ameliorates ovarian dysfunction by regulating autophagy in PCOS via the PI3K-Akt pathway

    DOI: 10.1530/REP-20-0643

    PMID: 33989172

    		 Sample: Ovary
  48. Annals of Translational Medicine (2022) IF: 3.932
    The method of Yiqi Yangyin Tongluo can attenuate the pyroptosis of rheumatoid arthritis chondrocytes through the ASIC1a/NLRP3 signaling pathway

    DOI: 10.21037/atm-21-6822

    PMID: 35284559

    		 Sample: serum
  49. Biomed Research International (2019) IF: 2.197
    In Vivo, Proteomic, and In Silico Investigation of Sapodilla for Therapeutic Potential in Gastrointestinal Disorders

    DOI: 10.1155/2019/4921086
    Sample: gastric tissue
  50. Biomed Research International (2019) IF: 2.197
    Galangin Suppresses Renal Inflammation via the Inhibition of NF-κB, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells

    DOI: 10.1155/2019/3018357

    PMID: 31240210

    		 Sample: Cell culture supernatant
  51. FEBS Open Bio (2020) IF: 2.231
    ASIC1a inhibits cell pyroptosis induced by acid‐induced activation of rat hepatic stellate cells

    DOI: 10.1002/2211-5463.12850

    PMID: 32237041

    		 Sample: Cell culture medium
  52. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Emodin ameliorates acute pancreatitis‑induced lung injury by suppressing NLRP3 inflammasome‑mediated neutrophil recruitment

    DOI: 10.3892/etm.2021.10289
    Sample: serum
  53. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Activated protein C overexpression suppresses the pyroptosis of subarachnoid hemorrhage model cells by regulating the NLRP3 inflammasome pathway

    DOI: 10.3892/etm.2021.10827

    PMID: 34650639

    		 Sample: brain tissue
  54. Journal of Ovarian Research (2019) IF: 2.469
    Abnormal expression of HSP70 may contribute to PCOS pathology

    DOI: 10.1186/s13048-019-0548-7
    Sample: serum
  55. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Proteomic Analysis and In Vivo Studies Reveal the Potential Gastroprotective Effects of CHCl3 and Aqueous Extracts of Ficus palmata

    DOI: 10.1155/2021/6613140
    Sample: Tissue homogenate
  56. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Moxibustion against Cyclophosphamide-Induced Premature Ovarian Failure in Rats through Inhibiting NLRP3-/Caspase-1-/GSDMD-Dependent Pyroptosis

    DOI: 10.1155/2021/8874757

    PMID: 33613687

    		 Sample: Serum
  57. EXPERIMENTAL BIOLOGY AND MEDICINE (2020) IF: 2.691
    Protective effect of exogenous hydrogen sulfide on diaphragm muscle fibrosis in streptozotocin-induced diabetic rats:

    DOI: 10.1177/1535370220931038
    Sample: Tissue homogenate
  58. Experimental and Therapeutic Medicine (2022) IF: 2.751
    S100A9‑containing serum exosomes obtained from patients with burn injuries promote myocardial cell pyroptosis through NLRP3

    DOI: 10.3892/etm.2022.11583

    PMID: 36277145

    		 Sample: AC16 cell
  59. Radiation Oncology (2020) IF: 2.817
    Hippocampal changes in inflammasomes, apoptosis, and MEMRI after radiation-induced brain injury in juvenile rats

    DOI: 10.1186/s13014-020-01525-3

    PMID: 32276638

    		 Sample: Tissue homogenate
  60. ACS Omega (2020) IF: 2.87
    Hydroxysafflor Yellow A Together with Blood–Brain Barrier Regulator Lexiscan for Cerebral Ischemia Reperfusion Injury Treatment

    DOI: 10.1021/acsomega.0c02502
    Sample: Tissue homogenate
  61. MOLECULAR AND CELLULAR BIOCHEMISTRY (2019) IF: 2.884
    The protective effect of cannabinoid type 2 receptor activation on renal ischemia–reperfusion injury

    DOI: 10.1007/s11010-019-03616-6

    PMID: 31446615

    		 Sample: Serum
  62. Molecular Medicine Reports (2022) IF: 2.952
    SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β‑catenin/TCF/LEF signaling pathway

    DOI: 10.3892/mmr.2022.12709

    PMID: 35419615

    		 Sample: Normal rat hepatocytes (BRL)
  63. JOURNAL OF CELLULAR BIOCHEMISTRY (2018) IF: 2.959
    Protective effect of N‐(p‐amylcinnamoyl) anthranilic acid, phospholipase A2 enzyme inhibitor, and transient receptor potential melastatin‐2 channel blocker against renal ischemia–reperfusion injury

    DOI: 10.1002/jcb.27664
    Sample: Serum
  64. Journal of Ovarian Research (2020) IF: 2.989
    The effect of glutamine on Dehydroepiandrosterone-induced polycystic ovary syndrome rats.

    DOI: 10.1186/s13048-020-00650-7
    Sample: Serum
  65. Acta Cirurgica Brasileira (2018) IF: 1.388
    Effect of ozone oxidative preconditioning on inflammation and oxidative stress injury in rat model of renal transplantation

    DOI: 10.1590/s0102-865020180030000006
    Sample: Serum
  66. Experimental and Therapeutic Medicine (2018) IF: 1.41
    Thymoquinone reduces spinal cord injury by inhibiting inflammatory response, oxidative stress and apoptosis via PPAR‑γ and PI3K/Akt pathways

    DOI: 10.3892/etm.2018.6072
    Sample: Tissue homogenate
  67. Molecular & Cellular Toxicology (2022) IF: 1.718
    Efficacy of urinary trypsin inhibitor in the treatment of rats with severe sepsis and its effects on coagulation and immunity

    DOI: 10.1007/s13273-022-00303-4
    Sample: hippocampus
  68. Molecular Medicine Reports (2019) IF: 1.851
    MicroRNA‑22 alleviates inflammation in ischemic stroke via p38 MAPK pathways

    DOI: 10.3892/mmr.2019.10269
    Sample: serum
  69. MEDICAL SCIENCE MONITOR (2020) IF: 1.918
    Carbon Monoxide Inhibits the Expression of Proteins Associated with Intestinal Mucosal Pyroptosis in a Rat Model of Sepsis Induced by Cecal Ligation and Puncture

    DOI: 10.12659/MSM.920668
    Sample: serum,intestinal tissue
  70. MEDICAL SCIENCE MONITOR (2019) IF: 1.98
    Electroacupuncture Alleviates Ischemic Brain Injury by Inhibiting the miR-223/NLRP3 Pathway

    DOI: 10.12659/MSM.917213
    Sample: cortical
  71. International Journal of Fertility & Sterility (2022)
    Silver Nanoparticles Biofabricated from Cinnamomum zeylanicum Reduce IL-6, IL-18, and TNF-ɑ in Female Rats with Polycystic Ovarian Syndrome

    DOI: 10.22074/IJFS.2022.539396.1189
    Sample: serum
  72. RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY (2018) IF: 0.796
    Dopamine Pretreatment Protects Offspring Rats from LPS-Induced Hypertension and Kidney Damage by Inhibiting NLRP3 Activation in Kidney

    DOI: 10.1134/S1068162018010077
    Sample: serum
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