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Rat NFKB-p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

Cat.No.:E-EL-R0673
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat NFKB-p105 in samples. No significant cross-reactivity or interference between Rat NFKB-p105 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat NFKB-p105 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NFKB-p105. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NFKB-p105 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NFKB-p105, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NFKB-p105. You can calculate the concentration of Rat NFKB-p105 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NFKB-p105 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NFKB-p105 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.47	1.42	4.64	0.51	1.36	4.23
Standard deviation	0.03	0.06	0.21	0.03	0.07	0.17
CV (%)	6.38	4.23	4.53	5.88	5.15	4.02

Recovery

The recovery of Rat NFKB-p105 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-103	96
EDTA plasma (n=8)	92-106	98
Cell culture media (n=8)	86-99	92

Linearity

Samples were spiked with high concentrations of Rat NFKB-p105 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	NFKB1, NF-KB1, NF-Kappa-B, NFKB-p105, NFKB-p50, p105, p50, EBP-1, KBF1
Research Area	Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Immunology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
Glutamine restores testicular glutathione-dependent antioxidant defense and upregulates NO/cGMP signaling in sleep deprivation-induced reproductive dysfunction in rats

DOI: 10.1016/j.biopha.2022.112765

PMID: 35247715
Sample: Testicular,epididymal
LIFE SCIENCES (2021) IF: 5.037
Protective effects of hesperidin through attenuation of Ki67 expression against DMBA-induced breast cancer in female rats

DOI: 10.1016/j.lfs.2021.119957

PMID: 34530017
Sample: Breast tissue
FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 5.572
Banana peel ameliorated hepato-renal damage and exerted anti-inflammatory and anti-apoptotic effects in metal mixture mediated hepatic nephropathy by activation of Nrf2/ Hmox-1 and inhibition of Nfkb pathway

DOI: 10.1016/j.fct.2022.113471

PMID: 36270409
Sample: liver,kidney
BRAIN RESEARCH BULLETIN (2021) IF: 4.079
Effect of lobeglitazone on motor function in rat model of Parkinson’s disease with diabetes co-morbidity

DOI: 10.1016/j.brainresbull.2021.05.011

Sample: Tissue homogenate
Journal of Traditional and Complementary Medicine (2021) IF: 4.221
Menhaden fish oil attenuates postpartum depression in rat model via inhibition of NLRP3-inflammasome driven inflammatory pathway

DOI: 10.1016/j.jtcme.2021.02.007

Sample: brain
INFLAMMOPHARMACOLOGY (2021) IF: 4.473
Dimethyl fumarate attenuates 2-VO-induced vascular dementia via activating the Nrf2 signaling pathway in rats

DOI: 10.1007/s10787-020-00785-5

PMID: 33459879
Sample: Tissue homogenate(hippocampus)
TOXICOLOGY (2022) IF: 4.571
Zinc and selenium mitigated heavy metals mixture (Pb, Al, Hg and Mn) mediated hepatic-nephropathy via modulation of oxido-inflammatory status and NF‑kB signaling in female albino rats

DOI: 10.1016/j.tox.2022.153350

PMID: 36220500
Sample: liver,kidney
NEUROSCIENCE (2023) IF: 3.708
Essential Trace Elements Prevent the Impairment in the Retention Memory, Cerebral Cortex, and Cerebellum Damage in Male Rats Exposed to Quaternary Metal Mixture by Up-regulation, of Heme Oxygynase-1 and Down-regulation of Nuclear Factor Erythroid 2-relate

DOI: 10.1016/j.neuroscience.2023.01.002

PMID: 36646412
Sample: cerebral cortex,cerebellum
ENDOCRINE (2022) IF: 3.925
Acetate restores hypothalamic-adipose kisspeptin status in a rat model of PCOS by suppression of NLRP3 immunoreactivity

DOI: 10.1007/s12020-022-03191-9

PMID: 36114434
Sample: hypothalamic tissue,VAT
NEUROCHEMICAL RESEARCH (2022) IF: 3.996
Tetramethylpyrazine Attenuates Cognitive Impairment Via Suppressing Oxidative Stress, Neuroinflammation, and Apoptosis in Type 2 Diabetic Rats

DOI: 10.1007/s11064-022-03640-x

PMID: 35665448
Sample: hippocampal tissue
Metabolic Syndrome and Related Disorders (2022) IF: 2.363
Naringenin Prevents Inflammation, Apoptosis, and DNA Damage in Potassium Oxonate-Induced Hyperuricemia in Rat Liver Tissue: Roles of Cytochrome C, NF-κB, Caspase-3, and 8-Hydroxydeoxyguanosine

DOI: 10.1089/met.2022.0028

PMID: 35796694
Sample: Liver tissue
PHARMACEUTICAL BIOLOGY (2020) IF: 2.971
Protective effect of apigenin magnesium complex on H2O2-induced oxidative stress and inflammatory responses in rat hepatic stellate cells

DOI: 10.1080/13880209.2020.1772840

Sample: Cell culture supernatant
Pharmacognosy Magazine (2017) IF: 1.069
The Effect of Thymoquinone on Nuclear Factor Kappa B Levels and Oxidative DNA Damage on Experimental Diabetic Rats

DOI: 10.4103/pm.pm_134_17

Sample: serum
Journal of Oleo Science (2022) IF: 1.628
Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

DOI: 10.5650/jos.ess22033

PMID: 35965085
Sample: liver
Journal of Diabetes and Metabolic Disorders (2023)
Effect of Oleanolic acid administration on hepatic AMPK, SIRT-1, IL-6 and NF-κB levels in experimental diabetes

DOI: 10.1007/s40200-022-01178-x

Sample: Liver tissue

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