Rat NGAL (Neutrophil Gelatinase Associated Lipocalin) CLIA Kit
Price: $ 682
Price: $ 546
Price: $ 180
Price: Inquire
Price: Inquire
Size:
96T
48T
24T
96T*10
96T*5
Quantity:
- Reactivity: Rat
- Detection Range: 15.63-1000 pg/mL
- Sensitivity: 9.38 pg/mL
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Product Details
Test Principle | This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NGAL. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat NGAL. You can calculate the concentration of Rat NGAL in the samples by comparing the RLU value of the samples to the standard curve. |
Assay Type | Sandwich-ELISA |
size | 96T / 48T / 24T / 96T*10 / 96T*5 |
Assay Time | 3.5h |
Detection Method | Chemiluminescence |
Detection Range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample Volume | 100μL |
Sample Type | serum, plasma and other biological fluids |
Specificity | This kit recognizes Rat NGAL in samples.No significant cross-reactivity or interference between Rat NGAL and analogues was observed |
Precision | Both intra-CV and inter-CV are < 10%. |
Recovery | 80%-120% |
Introduction | This ELISA kit applies to the in vitro quantitative determination of Rat NGAL concentrations in serum, plasma and other biological fluids. |
Applications | CLIA |
Storage | 2-8℃/-20℃ |
Target Information
Database | SwissProt: P30152 |
Syonoyms | LCN2,Lipocalin 2,Oncogene 24p3,MSFI |
Research Area | Cancer , Cardiovascular , Metabolism , Immunology , Signal Transduction |
Assay Procedures
Citations
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Pentoxifylline and berberine mitigate diclofenac-induced acute nephrotoxicity in male rats via modulation of inflammation and oxidative stress
IF:6.53
Journal:BIOMEDICINE & PHARMACOTHERAPY
DOI:10.1016/j.biopha.2022.113225
PMID:35671584
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Candesartan Protects Against Cadmium-Induced Hepatorenal Syndrome by Affecting Nrf2, NF-κB, Bax/Bcl-2/Cyt-C, and Ang II/Ang 1–7 Signals
IF:3.738
Journal:BIOLOGICAL TRACE ELEMENT RESEARCH