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Rat NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA Kit

Citations(5)Uniprot : P29476

Cat.No.:E-EL-R1438
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat NOS1/nNOS in samples. No significant cross-reactivity or interference between Rat NOS1/nNOS and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat NOS1/nNOS concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS1/nNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS1/nNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS1/nNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NOS1/nNOS. You can calculate the concentration of Rat NOS1/nNOS in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NOS1/nNOS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NOS1/nNOS were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.60	4.50	0.50	1.40	4.70
Standard deviation	0.03	0.07	0.19	0.03	0.08	0.15
CV (%)	6.00	4.38	4.22	6.00	5.71	3.19

Recovery

The recovery of Rat NOS1/nNOS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-98	92
EDTA plasma (n=8)	88-101	95
Cell culture media (n=8)	85-96	90

Linearity

Samples were spiked with high concentrations of Rat NOS1/nNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P29476
Synonyms	bNOS, IHPS1
Research Area	Cancer, Metabolism, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

PHYTOMEDICINE (2022) IF: 6.656
A discovery of clinically approved Panlongqi Tablet for repositioning to treat osteoarthritis by inhibiting PI3K/AKT activation

DOI: 10.1016/j.phymed.2022.154360

Sample: serum
EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
Nitric oxide modulates tapentadol antinociceptive tolerance and physical dependence

DOI: 10.1016/j.ejphar.2021.174245

PMID: 34126091
Sample: lumbar
BEHAVIOURAL BRAIN RESEARCH (2022) IF: 3.352
Prenatal chronic stress impairs the learning and memory ability via inhibition of the NO/cGMP/PKG pathway in the Hippocampus of offspring

DOI: 10.1016/j.bbr.2022.114009

PMID: 35850398
Sample: hippocampal tissue
MOLECULAR VISION (2018) IF: 2.219
Protective effect of magnesium acetyltaurate and taurine against NMDA-induced retinal damage involves reduced nitrosative stress

PMID: 30090013
Sample: retinal tissues
Annals of Surgical Treatment and Research (2019) IF: 1.181
Examination of protective and therapeutic effects of ruscogenin on cerulein-induced experimental acute pancreatitis in rats

DOI: 10.4174/astr.2019.97.6.271

PMID: 31824881
Sample: Serum,Tissue homogenate

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