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Rat SDC1(Syndecan 1) ELISA Kit

Cat.No.:E-EL-R0996
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat SDC1 in samples. No significant cross-reactivity or interference between Rat SDC1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat SDC1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SDC1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SDC1. You can calculate the concentration of Rat SDC1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat SDC1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat SDC1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.89	14.44	42.98	5.15	14.33	44.03
Standard deviation	0.26	0.60	1.82	0.32	0.80	1.39
CV (%)	5.32	4.16	4.23	6.21	5.58	3.16

Recovery

The recovery of Rat SDC1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-103	97
EDTA plasma (n=8)	91-107	98
Cell culture media (n=8)	93-106	99

Linearity

Samples were spiked with high concentrations of Rat SDC1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	CD138, SDC, SYND1,Syndecan 1
Research Area	Cancer, Metabolism, Neuroscience, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BRITISH JOURNAL OF ANAESTHESIA (2021) IF: 9.166
Bosutinib reduces endothelial permeability and organ failure in a rat polytrauma transfusion model

DOI: 10.1016/j.bja.2021.01.032

PMID: 33685634
Sample: Whole Blood
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
The Role of IGL-2 Preservation Solution on Rat Livers during SCS and HOPE

DOI: 10.3390/ijms232012615

PMID: 36293465
Sample: livers
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208
Protective Effect of Oxygen and Isoflurane in Rodent Model of Intestinal Ischemia-Reperfusion Injury

DOI: 10.3390/ijms24032587

Sample: Plasma
Blood Advances (2021) IF: 6.799
Plasma and rhADAMTS13 reduce trauma-induced organ failure by restoring the ADAMTS13-VWF axis

DOI: 10.1182/bloodadvances.2021004404

Sample: plasma
Scientific Reports (2021) IF: 4.38
Validation of the relationship between coagulopathy and localization of hydroxyethyl starch on the vascular endothelium in a rat hemodilution model

DOI: 10.1038/s41598-021-89889-8

PMID: 34021192
Sample: Plasma
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2018) IF: 3.687
Glycocalyx Preservation and NO Production in Fatty Livers—The Protective Role of High Molecular Polyethylene Glycol in Cold Ischemia Injury

DOI: 10.3390/ijms19082375

Sample: Tissue homogenate
TRANSFUSION (2020) IF: 2.8
The use of cryopreserved platelets in a trauma-induced hemorrhage model

DOI: 10.1111/trf.15937

PMID: 32592423
Sample: Tissue homogenate
Dubai Medical Journal (2022)
Fluid Resuscitation Aggravates the Cellular Injury in a Hemorrhagic Shock Model

DOI: 10.1159/000520430

Sample: serum

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