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Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

Citations(35)Uniprot : P07632

  • Cat.No.:E-EL-R1424

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes SOD1 in samples. No significant cross-reactivity or interference between SOD1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of SOD1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SOD1. During the reaction, SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SOD1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SOD1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.20 3.40 0.50 1.30 3.60
Standard deviation 0.03 0.07 0.12 0.03 0.07 0.13
CV (%) 6.00 5.83 3.53 6.00 5.38 3.61

Recovery

The recovery of SOD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 97
EDTA plasma(n=8) 91-104 97
Cell culture media(n=8) 89-105 96

Linearity

Samples were spiked with high concentrations of Rat SOD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P07632
SynonymsALS, ALS1, IPOA, Superoxide dismutase [Cu-Zn]
Research AreaCancer, Cell Biology, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
elisa assay procedure 2

2. Aspirate and wash the plate for 3 times
elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 5

5. Add 50μL Stop Solution
elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Biomedicines (2021) IF: 6.081
    WIN55,212-2 Attenuates Cognitive Impairments in AlCl3 + d-Galactose-Induced Alzheimer’s Disease Rats by Enhancing Neurogenesis and Reversing Oxidative Stress

    DOI: 10.3390/biomedicines9091270
    Sample: brain
  2. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Pentoxifylline and berberine mitigate diclofenac-induced acute nephrotoxicity in male rats via modulation of inflammation and oxidative stress

    DOI: 10.1016/j.biopha.2022.113225

    PMID: 35671584

    		 Sample: serum
  3. INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY (2019) IF: 5.176
    Emodin Rescued Hyperhomocysteinemia-Induced Dementia and Alzheimer’s Disease-Like Features in Rats

    DOI: 10.1093/ijnp/pyy090
    Sample: Plasma,Tissue homogenate
  4. RNA Biology (2019) IF: 5.477
    MicroRNA-142-3p suppresses endometriosis by regulating KLF9-mediated autophagy in vitro and in vivo

    DOI: 10.1080/15476286.2019.1657352

    PMID: 31425004

    		 Sample: Serum,Cell culture medium
  5. Frontiers in Pharmacology (2021) IF: 5.811
    The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

    DOI: 10.3389/fphar.2021.709702
    Sample: serum
  6. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Centella asiatica extract protects against cisplatin-induced hepatotoxicity via targeting oxidative stress, inflammation, and apoptosis

    DOI: 10.1007/s11356-022-18626-z

    PMID: 35029831

    		 Sample: hepatic tissue
  7. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
    Protection of melatonin against acidosis‐induced neuronal injuries

    DOI: 10.1111/jcmm.15351
    Sample: Primary cortical neurons
  8. Nutrients (2020) IF: 4.546
    Effect of Defatted Dabai Pulp Extract in Urine Metabolomics of Hypercholesterolemic Rats

    DOI: 10.3390/nu12113511

    PMID: 33202660

    		 Sample: Serum
  9. BIOFACTORS (2020) IF: 4.734
    Berberine promotes peri‐implant osteogenesis in diabetic rats by ROS‐mediated IRS‐1 pathway

    DOI: 10.1002/biof.1692
    Sample: serum
  10. NEUROSCIENCE LETTERS (2021) IF: 3.046
    Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

    DOI: 10.1016/j.neulet.2021.136385

    PMID: 34871743

    		 Sample: hippocampus,liver
  11. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2021) IF: 3.168
    Heamanetic Effects of a Dioxidovanadium(V) Complex in STZ-Induced Diabetic Male Sprague Dawley Rats

    DOI: 10.2147/DMSO.S214726

    PMID: 34707382

    		 Sample: plasma
  12. PLoS One (2021) IF: 3.24
    A low direct electrical signal attenuates oxidative stress and inflammation in septic rats

    DOI: 10.1371/journal.pone.0257177

    PMID: 34499695

    		 Sample: serum,Lung tissue
  13. TOXICOLOGY (2018) IF: 3.265
    Fluoride induced tissue hypercalcemia, IL-17 mediated inflammation and apoptosis lead to cardiomyopathy: Ultrastructural and biochemical findings

    DOI: 10.1016/j.tox.2018.05.012
    Sample: Tissue homogenate
  14. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2019) IF: 3.319
    Plant-derived oleanolic acid ameliorates markers associated with non-alcoholic fatty liver disease in a diet-induced pre-diabetes rat model

    DOI: 10.2147/DMSO.S218626
    Sample: plasma
  15. Frontiers in Behavioral Neuroscience (2022) IF: 3.558
    Acupuncture Relieves Stress-Induced Depressive Behavior by Reducing Oxidative Stress and Neuroapoptosis in Rats

    DOI: 10.3389/fnbeh.2021.783056

    PMID: 35058758

    		 Sample: hippocampus tissue
  16. BMC Complementary and Alternative Medicine (2022) IF: 3.659
    Attenuation of carbohydrate metabolism and lipid profile by methanolic extract of Euphorbia helioscopia and improvement of beta cell function in a type 2 diabetic rat model

    DOI: 10.1186/s12906-022-03507-2

    PMID: 35078449

    		 Sample: serum
  17. BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
    The effects of Momordica balsamina methanolic extract on haematological function in streptozotocin-induced diabetic rats: Effects on selected markers

    DOI: 10.1016/j.biopha.2019.108925
    Sample: plasma,kidney tissues
  18. NEUROCHEMISTRY INTERNATIONAL (2021) IF: 3.921
    Exposure to footshock stress downregulates antioxidant genes and increases neuronal apoptosis in an Aβ(1–42) rat model of Alzheimer's disease

    DOI: 10.1016/j.neuint.2021.105170
  19. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2019) IF: 2.041
    The effect of dioxidovanadium complex (V) on hepatic function in streptozotocin-induced diabetic rats

    DOI: 10.1139/cjpp-2019-0369

    PMID: 31491333

    		 Sample: Serum,Plasma,Tissue homogenate
  20. BMC Cardiovascular Disorders (2022) IF: 2.174
    Assessing the risk factors for myocardial infarction in diet-induced prediabetes: myocardial tissue changes

    DOI: 10.1186/s12872-022-02758-8

    PMID: 35918636

    		 Sample: heart tissue
  21. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2021) IF: 2.273
    Anti-hyperglycaemic effects of dioxidovanadium complex cis-[VO2(obz)py] avert kidney dysfunction in streptozotocin-induced diabetic male Sprague–Dawley rats

    DOI: 10.1139/cjpp-2020-0278
    Sample: kidney
  22. BIOLOGICAL TRACE ELEMENT RESEARCH (2020) IF: 2.639
    Apoptotic and Degenerative Changes in the Enteric Nervous System Following Exposure to Fluoride During Pre- and Post-natal Periods

    DOI: 10.1007/s12011-020-02249-x

    PMID: 32594358

    		 Sample: Tissue homogenate
  23. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
    San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

    DOI: 10.1155/2022/7832540
    Sample: hepatic tissue
  24. Dose-Response (2022) IF: 2.658
    Pharmacological Evaluation of Mentha piperita Against Urolithiasis: An In Vitro and In Vivo Study:

    DOI: 10.1177/15593258211073087
    Sample: kidney
  25. MICROSCOPY RESEARCH AND TECHNIQUE (2021) IF: 2.769
    Histological, immunohistochemical, and molecular investigation on the hepatotoxic effect of potassium dichromate and the ameliorating role of Persea americana mill pulp extract

    DOI: 10.1002/jemt.23798

    PMID: 33908126

    		 Sample: Tissue homogenate(liver)
  26. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2020) IF: 2.802
    Curcumin-loaded cockle shell-derived calcium carbonate nanoparticles: A novel strategy for the treatment of lead-induced hepato-renal toxicity in rats

    DOI: 10.1016/j.sjbs.2020.03.009

    PMID: 32489292

    		 Sample: Serum
  27. BRAIN RESEARCH (2019) IF: 2.929
    Effect of enriched environment and predictable chronic stress on spatial memory in adolescent rats: Predominant expression of BDNF, nNOS, and interestingly malondialdehyde in the right hippocampus

    DOI: 10.1016/j.brainres.2019.146326
    Sample: hippocampus
  28. Molecular Medicine Reports (2018) IF: 2.952
    Puerarin reduces ischemia/reperfusion-induced myocardial injury in diabetic rats via upregulation of vascular endothelial growth factor A/angiotensin-1 and suppression of apoptosis

    DOI: 10.3892/mmr.2018.8754
    Sample: plasma
  29. Experimental and Therapeutic Medicine (2017) IF: 1.261
    Tocotrienol alleviates inflammation and oxidative stress in a rat model of spinal cord injury via suppression of transforming growth factor-β

    DOI: 10.3892/etm.2017.4505
    Sample: Serum
  30. Anatolian Journal of Cardiology (2018) IF: 1.271
    Protective effects of curcumin and beta-carotene on cisplatin-induced cardiotoxicity: An experimental rat model

    DOI: 10.14744/AnatolJCardiol.2018.53059
    Sample: Tissue homogenate
  31. Molecular Medicine Reports (2015) IF: 1.554
    Asiaticoside attenuates the effects of spinal cord injury through antioxidant and anti‑inflammatory effects, and inhibition of the p38‑MAPK mechanism

    DOI: 10.3892/mmr.2015.4425
    Sample: Serum
  32. ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS (2022) IF: 1.753
    Protective effect by low-intensity downhill running training against muscle damage and oxidative stress after high-intensity downhill running in rats

    DOI: 10.1590/0001-3765202220200265

    PMID: 35507975

    		 Sample: Plasma,vastus intermedius muscle tissue
  33. Brazilian Journal of Otorhinolaryngology (2022) IF: 1.811
    Astaxanthin protects against hearing impairment in diabetic rats

    DOI: 10.1016/j.bjorl.2022.02.004

    PMID: 35331657

    		 Sample: serum,cochlea
  34. INTERNATIONAL UROLOGY AND NEPHROLOGY (2020) IF: 1.843
    Vinpocetine reduces cisplatin-induced acute kidney injury through inhibition of NF–κB pathway and activation of Nrf2/ARE pathway in rats

    DOI: 10.1007/s11255-020-02485-z
    Sample: Serum,Urine,Tissue homogenate
  35. Advances in Traditional Medicine (2020)
    Tualang honey and its methanolic fraction ameliorate lipopolysaccharide-induced oxidative stress, amyloid deposition and neuronal loss of the rat hippocampus

    DOI: 10.1007/s13596-020-00449-3
    Sample: Tissue homogenate
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Reviews/Q&A

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  • Q&A
Q

E********bSubmitted [ Dec 17 2020 ]

Asked: What species can Catalog.No: E-EL-R1424 be used? Rat, mouse? thank you

Reply

A

adminSubmitted [ Dec 17 2020 ]

Answered: Hi Elly, Thanks for your message. E-EL-R1424 detects Rat samples

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