Current Position:Home>>Kits>>ELISA Kits>> Rat VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit
Cat.No.:E-EL-R2603
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Assay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 31.25-2000 pg/mL |
Sensitivity | 18.75 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Rat VEGF-A in samples. No significant cross-reactivity or interference between Rat VEGF-A and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Rat VEGF-A concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat VEGF-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat VEGF-A were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 97.90 | 170.39 | 948.99 | 89.62 | 158.20 | 968.59 |
Standard deviation | 5.02 | 9.25 | 46.60 | 5.65 | 7.26 | 37.97 |
CV (%) | 5.13 | 5.43 | 4.91 | 6.30 | 4.59 | 3.92 |
The recovery of Rat VEGF-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 86-101 | 93 |
EDTA plasma (n=8) | 90-102 | 95 |
Cell culture media (n=8) | 89-101 | 95 |
Samples were spiked with high concentrations of Rat VEGF-A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Research Area | Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
PMID: 33842738 |
Sample: Cell culture supernatant |
Sample: neural stem cells (NSCs) |
PMID: 36189833 |
Sample: BMSCs |
Sample: Cell culture supernatant |
Sample: bone marrow mesenchymal stem cells (BMSCs) |
Sample: EPCs |
PMID: 35625715 |
Sample: liver tissue |
DOI: 10.3390/ijms24032544 |
|
Sample: retinal microvascular pericytes (RMPs) |
DOI: 10.1155/2022/7319563 |
|
PMID: 35578728 |
Sample: brain |
PMID: 36334391 |
Sample: serum |
PMID: 36228770 |
Sample: serum |
PMID: 32456711 |
Sample: Plasma |
PMID: 33899465 |
Sample: Tissue homogenate,Serum |
PMID: 35526592 |
Sample: C6 cell,U-87 MG cell,LN229 cell |
PMID: 31425004 |
Sample: Serum,Cell culture medium |
PMID: 34630092 |
Sample: hippocampal tissue |
Sample: heart tissue |
DOI: 10.1002/tox.23639 |
|
Sample: serum,NR8383 cell |
PMID: 34748867 |
Sample: eyes |
PMID: 34961769 |
Sample: serum |
DOI: 10.1242/jcs.216135 |
|
Sample: Tissue homogenate |
PMID: 33031799 |
Sample: Serum |
DOI: 10.2147/IJN.S184920 |
|
PMID: 30666115 |
Sample: Cell culture supernatant |
PMID: 34091811 |
Sample: ankle |
PMID: 35412166 |
Sample: paw tissue |
DOI: 10.1055/a-1997-5566 |
|
Sample: burn wound tissue |
PMID: 31550444 |
Sample: Cell lysate |
Sample: IEC-6 cell |
Sample: Serum |
Sample: VEGF solution after immobilization |
Sample: Tissue homogenate |
Sample: Tissue homogenate |
Sample: Serum |
Sample: serum |
Sample: gastric tissue. |
DOI: 10.1155/2022/4116563 |
|
PMID: 35069758 |
Sample: skin |
Sample: serum |
Sample: serum |
PMID: 31938037 |
Sample: Serum,Cell culture medium |
Sample: corneal tissue |
Sample: EPCs cells |
DOI: 10.1111/jfbc.13258 |
|
Sample: serum |
DOI: 10.1111/and.13472 |
|
Sample: Serum |
DOI: 10.17392/1208-20 |
|
Sample: blood |
Sample: IEC-6 cell |
Sample: serum |
Sample: Serum |