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Recombinant 2019-nCoV Guanine-N7_meth Protein (His Tag)

Uniprot : YP_009725309.1

Cat.No.:PKSR030473
Expression host: E.coli

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Description

Synonyms	SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase
Species	Virus
Expression_host	E.coli
Sequence	Ala1-Gln527
Accession	YP_009725309.1
Mol_Mass	62.9 kDa
AP_Mol_Mass	60 kDa
Tag	N-6His

Properties

Purity	> 85 % as determined by reducing SDS-PAGE.
Endotoxin level	Please contact us for more information.
Storage	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Formulation	Supplied as a 0.2 μM filtered solution of PBS, 10% Glycerol, pH 7.4.
Reconstitution	Please refer to the printed manual for detailed information.

Background

The nonstructural protein (nsp) 14 of 2019-nCoV was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). 2019-nCoV polyproteins incorporate 16 protein domains (nsps). The putative non-structural protein 2 (nsp2) of SARS-CoV plays an important role in viral transcription and replication, and is an attractive target for anti-SARS drug development.

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Reviews/Q&A

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Reviews
Q&A

J***************aSubmitted [ Mar 25 2022 ]

Asked: Could you please provide the exact concentration and the volume (mg/ml) of the 10ug size nsp14 sample (PKSR030473). Thanks

adminSubmitted [ Mar 25 2022 ]

Answered: Hi Jolanta, the concentration of the current batch of this item is 0.48mg/ml. Any questions please feel free to contact us. Thank you.

A************ Submitted [ Dec 01 2020 ]

Asked: Is the protein preparation tested for enzymatic activity ?

adminSubmitted [ Dec 01 2020 ]

Answered: Hi Angela, Thanks for your message. The nonstructural protein (nsp) 14 of 2019-nCoV was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Could you let us know which country you are from, so that I could forward to our local sales representative to contact you ASAP. Best regards

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