Recombinant SARS-CoV-2 Nucleoprotein / NP Protein (His Tag)
Price: $ 1105
Size:
100μg
Quantity:
- Expression Host: Baculovirus-Insect Cells
- Accession: NP_828858.1
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For research use only. Order now, ship in 3 days
Product Details
Synonyms | 2019-nCoV coronavirus NP Protein;2019-nCoV np Protein;2019-nCoV novel coronavirus Nucleoprotein Protein |
Species | SARS-CoV-2 |
Expression Host | Baculovirus-Insect Cells |
Sequence | Met1-Ala422 |
Accession | NP_828858.1 |
Calculated Molecular Weight | 47.5 kDa |
Observed Molecular Weight | 47.1 kDa |
Tag | C-His |
Purity | > 80 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation |
LyopHilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |