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Recombinant 2019-nCoV Nucleoprotein / NP Protein (His Tag)

Uniprot : NP_828858.1
  • Cat.No.:PKSR030506

  • Expression host: Baculovirus-Insect Cells

To Purchase PKSR030506

Size:
  • 100µg
Price: $1105
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Description

Synonyms 2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
Species virus
Expression_host Baculovirus-Insect Cells
Sequence Met1-Ala422
Accession NP_828858.1
Application Immunogen(E-AB-V1013,E-AB-V1014)
Mol_Mass 47.5 kDa
AP_Mol_Mass 47.1 kDa
Tag C-His

Properties

Purity > 80 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per µg as determined by the LAL method.
Storage Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
Reconstitution Please refer to the printed manual for detailed information.

Background

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

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