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Cat.No.:PKSR030511
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Synonyms | Papain-like Protease;PLpro;PL-PRO;pp1a;Replicase polyprotein 1a |
Species | SARS-CoV-2 |
Expression_host | E.coli |
Sequence | Glu1564-Val1880 |
Accession | YP_009725299.1 |
Mol_Mass | 36.8 kDa |
Tag | N-His |
Bio_Activity | Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC). The Specific Activity is > 80pmols/min/μg |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile 20 mM Tris 500mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |