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Cat.No.:PKSR030500
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Synonyms | 2019-nCov S protein; 2019-nCoV Spike glycoprotein; 2019-nCoV S glycoprotein |
Species | Virus |
Expression_host | HEK293 Cells |
Sequence | Arg319-Phe541 |
Accession | YP_009724390.1 |
Application | Immunogen(E-AB-V1006,E-AB-V1025) |
Mol_Mass | 51.5 kDa |
Tag | C-mFc |
Bio_Activity | 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) (Cat#PKSR030508) at 2μg/mL (100μL/well) can bind Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) (Cat#PKSR030500), the EC50 of PKSR030500 is 15-50 ng/mL. 2. Loaded Human ACE2 Protein (His tag) (Cat#PKSR030508) on SA Biosensor, can bind Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) (Cat#PKSR030500) with an affinity constant of 0.562 nM as determined by Octet RED System. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per µg as determined by the LAL method. |
Storage | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | LyopHilized from sterile PBS, pH 7.4. |
Reconstitution | Please refer to the printed manual for detailed information. |
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