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Cat.No.:PKSH031462
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Synonyms | EPO-R |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Met-Pro 250 |
Accession | NP_000112.1 |
Application | Functional ELISA |
Mol_Mass | 51.0 kDa |
AP_Mol_Mass | 55-60 kDa |
Tag | C-hFc |
Bio_Activity | 1. Immobilized CD131 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human EPOR with a linear range of 0.16-4 μg/ml. 2. Measured by its ability to inhibit Epodependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1-4 ng/ml in the presence of 0.1 U/mL Recombinant Human EPO. |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, 8% sucrose, 0.5% Tween-20, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |