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Cat.No.:PKSH032431
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Synonyms | Fibroblast Growth Factor 1;FGF-1;Acidic Fibroblast Growth Factor;aFGF;Endothelial Cell Growth Factor;ECGFHeparin-Binding Growth Factor 1;HBGF-1;FGF1;FGFA |
Species | Human |
Expression_host | E.coli |
Sequence | Phe16-Asp155 |
Accession | P05230 |
Application | Cell Culture |
Mol_Mass | 16.8 kDa |
AP_Mol_Mass | 18 kDa |
Tag | C-His |
Bio_Activity | Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is <0.3 ng/mL. The specific activity of recombinant human FGF-1 is > 1 x 106 IU/mg. |
Purity | > 98 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 0.1 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS,pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |