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Recombinant Human FLT3LG/Flt3 Ligand Protein (His Tag)

Uniprot : P49771
  • Cat.No.:PKSH031692

  • Expression host: Baculovirus-Insect Cells

To Purchase PKSH031692

Size:
  • 10μg
  • 50μg
Price: $286
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Description

Synonyms Fms-Felated Tyrosine Kinase 3 Ligand;Flt3 Ligand;Flt3L;SL Cytokine;FLT3LG
Species Human
Expression_host Baculovirus-Insect Cells
Sequence Thr 27-Pro185
Accession P49771-1
Application Cell Culture
Mol_Mass 20.2 kDa
AP_Mol_Mass 27 kDa
Tag N-His
Bio_Activity Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3. The ED50 for this effect is typically 2-11 ng/mL.

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

FLT3L, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, FLT3L also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 -positive classical DCs and their CD103 -positive tissue counterparts.

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