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Recombinant Human Interleukin-3/IL-3 Protein (E.coli, His Tag)

Uniprot : P08700

Cat.No.:PKSH032639
Expression host: E.coli

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Description

Synonyms	Interleukin-3;IL-3;Hematopoietic Growth Factor;Mast Cell Growth Factor;MCGF;Multipotential Colony-Stimulating Factor;P-Cell-Stimulating Factor;IL3
Species	Human
Expression_host	E.coli
Sequence	Ala20-Phe152
Accession	AAC08706
Application	Cell Culture
Mol_Mass	16 kDa
AP_Mol_Mass	13 kDa
Tag	C-His
Bio_Activity	Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.15 ng/mL. The specific activity of recombinant human IL-3 is approximately >1.2 x 10⁶ IU/mg.

Properties

Purity	> 98 % as determined by reducing SDS-PAGE.
Endotoxin level	< 0.1 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Interleukin-3 (IL-3) is a potent growth promoting cytokine. IL-3 can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological function through binding to specific cell surface receptors. The amino acid sequences of this protein among different species share relatively low identity and its activity is highly species-specific. IL-3 has also been shown to possess neurotrophic activity; and is thought to be associated with neurologic disorders.

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