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Cat.No.:PKSH032906
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Synonyms | Platelet-Derived Growth Factor Subunit B;PDGF Subunit B;PDGF-2;Platelet-Derived Growth Factor B Chain;Platelet-Derived Growth Factor Beta Polypeptide;Proto-Oncogene c-Sis;Becaplermin;PDGFB;PDGF2;SIS |
Species | Human |
Expression_host | E.coli |
Sequence | Ser82-Thr190 |
Accession | P01127 |
Application | Cell Culture |
Mol_Mass | 12.4 kDa |
AP_Mol_Mass | 14 kDa |
Tag | None |
Bio_Activity | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 5-20 ng/ml. |
Purity | > 98 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 0.2 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |