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Cat.No.:PKSH031621
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Synonyms | Tumor necrosis factor receptor superfamily member 10D;Decoy receptor 2;DcR2;TNF-related apoptosis-inducing ligand receptor 4;TRAIL receptor 4;TRAIL-R4;TRAIL receptor with a truncated death domain;CD264;TNFRSF10D;DCR2;TRAILR4;TRUNDD |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Met 1-His 211 |
Accession | NP_003831.2 |
Application | Functional ELISA |
Mol_Mass | 18.4 kDa |
AP_Mol_Mass | 30-40 kDa |
Tag | C-His |
Bio_Activity | 1. Immobilized human TNFRSF10D at 10 μg/ml (100 μl/well) can bind biotinylated TNFSF10 with a linear range of 0.625-40 ng/ml. 2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 0.05-0.5 μg/mL in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10. |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |