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Cat.No.:PKSM040307
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Synonyms | Legumain;Lgmn;Asparaginyl endopeptidase;Protease cysteine 1;Prsc1;AEP |
Species | Mouse |
Expression_host | HEK293 Cells |
Sequence | Val 18-Tyr 435 |
Accession | NP_035305.1 |
Mol_Mass | 49.8 kDa |
AP_Mol_Mass | 55 kDa |
Tag | C-His |
Bio_Activity | Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC). The specific activity is > 350 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH) |
Purity | > 75 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile 25mM Tris, 0.15M NaCl, 20% Glycerol, pH 7.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |