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Cat.No.:PKSR030502
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Synonyms | S1 protein;2019-nCoV S1 protein;coronavirus S1 Protein;cov S1 Protein |
Species | SARS-CoV-2 |
Expression_host | HEK293 Cells |
Sequence | Val16-Arg685 |
Accession | YP_009724390.1 |
Mol_Mass | 101.7 kDa |
Tag | C-hFc |
Bio_Activity | 1. Immobilized human ACE2 protein (His tag) (Cat#PKSR030508) at 2μg/mL (100μL/well) can bind Recombinant 2019-nCoV S1 Protein (Fc Tag)(PKSR030502), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag) is 25-60 ng/mL. 2. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind Recombinant 2019-nCoV S1 Protein (Fc Tag) (PKSR030502), the EC50 of Recombinant 2019-nCoV S1 Protein (Fc Tag) is 25-60 ng/mL. |
Purity | > 85 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | LyopHilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |