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Uncoated Human HMGB-1(High Mobility Group Protein B1) ELISA Kit

Uniprot : P09429

Cat.No.:E-UNEL-H0065
Reactivity: Human

Applications:ELISA

To Purchase E-UNEL-H0065

Size:

96T*5
96T*15

Price: $399

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Application

The new series of Elabscience^® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.

For in vitro quantitative determination of HMGB-1 concentration in Human serum, plasma.

Properties

Assay type	Sandwich
Format	96T5 96T15
Assay time	24h
Reactivity	Human
Storage	-20℃. Please refer to the instructions for details.
Sample type	serum, plasma
Sample volume	100μL

Kit components

Item	Specifications	Storage
Micro ELISA pre-Plate(Dismountable)	96T*5：5 plates, 96T	-20℃, 6 months
Reference Standard	96T*5：1 vial
Concentrated Capture Ab	96T*5：1 vial 120μL
Concentrated Biotinylated Detection Ab	96T*5：1 vial 120μL
Concentrated HRP Conjugate	96T*5：1 vial 120μL	-20℃(Protect from light), 6 months
Product Description	1 copy	/
Certificate of Analysis	1 copy	/

Test Principle

	1. Analyte-specific antibody (capture antibody) is bound to a pre-microplate, unbound capture antibody is washed away. Then plates are blocked.
	2. Samples or standards are added and any analyte present is bound by the immobilized capture antibody. Unbound materials are decanted.
	3. The added Biotinylated Detection Ab will bind to the analyte been captured by the immobilized antibody. Unbound Biotinylated Detection Ab is washed away.
	4. HPR Conjugate is used to bind to the Biotinylated Detection Ab. Unbound HPR Conjugate is washed away.
	5. TMB substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Then the Stop Solution is added to stop the color change of substrate, and the color inside the well changes from blue to yellow. The absorbance of the color at 450 nm is measured.

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Reviews/Q&A

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Q&A

H***********nSubmitted [ Apr 23 2024 ]

Asked: Hi, can you please tell me the detection range and sensitivity of this assay? Which types of samples has it been tested with? Many thanks

adminSubmitted [ Apr 23 2024 ]

Answered: Dear Hannah Walden, the detection range for required item E-UNEL-H0065 is 31.25-2000 pg/mL. Moreover, for uncoated elisa kit, the sensitivity can be changed with the proportion of coated and detected antibodies, as well as the adjustment of the HRP enzyme conjugate, so you may need to adjust the experimental system according to the detection needs. We have verified Human serum/plasma samples. Best regards.

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