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Cat.No.:E-EL-0012
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Assay type | Competitive-ELISA |
Format | 96T/48T |
Assay time | 2.0h |
Detection range | 6.25-400 ng/mL |
Sensitivity | 3.75 ng/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 50μL |
Specificity | This kit recognizes VD in samples. No significant cross-reactivity or interference between VD and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of VD concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level VD were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level VD were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 21.62 | 53.66 | 157.91 | 21.41 | 57.30 | 152.81 |
Standard deviation | 1.28 | 2.70 | 5.98 | 1.47 | 2.35 | 8.34 |
CV (%) | 5.92 | 5.03 | 3.79 | 6.87 | 4.10 | 5.46 |
The recovery of VD spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 96-112 | 102 |
EDTA plasma(n=8) | 88-99 | 93 |
Cell culture media(n=8) | 85-97 | 91 |
Research Area | Metabolism |
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |
PMID: 36049670 |
Sample: serum |
PMID: 34478973 |
Sample: lung |
DOI: 10.5937/jomb0-30228 |
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Sample: serum |
DOI: 10.5578/tt.70027 |
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Sample: blood |
r****aSubmitted [ Nov 09 2022 ]
Asked: Can we use this kit for measuring mouse serum samples
Reply
adminSubmitted [ Nov 09 2022 ]
Answered: Yes,it is a universal kit and is suitable for mouse serum.
M******************ćSubmitted [ Mar 28 2022 ]
Asked: Does this kit measure levels of 25(OH) vitamin D in saliva samples?
Reply
adminSubmitted [ Mar 28 2022 ]
Answered:
D**uSubmitted [ Sep 25 2021 ]
Asked: Hi Does the Vitamin D (VD) ELISA kit ( E-EL-0012) measure calciferol (vitamin D 2 + vitamin D3) levels or 25 (OH) V D ?
Reply
adminSubmitted [ Sep 25 2021 ]
Answered: E-EL-0012 measures vitamin D 2 + vitamin D3 levels.
D*nSubmitted [ Sep 25 2021 ]
Asked: Hi Does this kit measure calciferol or 25 (OH) D?
Reply
adminSubmitted [ Sep 25 2021 ]
Answered: E-EL-0012 measures vitamin D 2 + vitamin D3 levels. E-EL-0015 measures 25 (OH) Vitamin D3 only.
D******aSubmitted [ Sep 25 2021 ]
Asked: Hi 1. Does this kit measure ( Vitamin D2 + Vitamin D3 ) levels or does it measure 25 (OH) D 3 levels? 2. What is the difference between this ELISA kit (E-EL-0012) and the 25 (OH) D3 ELISA kit (E-EL-0015)?
Reply
adminSubmitted [ Sep 25 2021 ]
Answered: E-EL-0012 measures vitamin D 2 + vitamin D3 levels. E-EL-0015 measures 25 (OH) Vitamin D3 only.
S*****eSubmitted [ Sep 25 2021 ]
Asked: Does this kit measure (Vitamin D3 + Vitamin D2) levels or 25 (OH) D3 levels?
Reply
adminSubmitted [ Sep 25 2021 ]
Answered: E-EL-0012 measures vitamin D 2 + vitamin D3 levels. E-EL-0015 measures 25 (OH) Vitamin D3 only.
E**eSubmitted [ Mar 26 2019 ]
Asked: Hello, I would like to ask what is evaluated in this test: Free vitamin D, Bioavailable vitamin D or 25(OH)D? Maybe you know the values of this test?
Reply
adminSubmitted [ Mar 26 2019 ]
Answered: Hi Egle, This kit detects 25(OH)Vintamin D, the reference values for human serum samples were 20-100 ng/mL.