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Cat.No.:E-EL-R1253
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Assay type | Sandwich-ELISA |
Format | 96T/48T/24 |
Assay time | 3.5h |
Detection range | 31.25-2000 pg/mL |
Sensitivity | 18.75 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Rat TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Rat TNNI3/cTn-I and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Rat TNNI3/cTn-I concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 12 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 12 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 12 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat TNNI3/cTn-I were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat TNNI3/cTn-I were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 102.40 | 248.30 | 900.40 | 97.00 | 237.90 | 894.70 |
Standard deviation | 5.70 | 14.90 | 36.90 | 5.10 | 11.40 | 37.60 |
CV (%) | 5.57 | 6.00 | 4.10 | 5.26 | 4.79 | 4.20 |
The recovery of Rat TNNI3/cTn-I spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 94-110 | 101 |
EDTA plasma (n=8) | 93-111 | 101 |
Cell culture media (n=8) | 93-107 | 100 |
Samples were spiked with high concentrations of Rat TNNI3/cTn-I and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | TNNC1, cTnI, CMD1FF, CMD2A, CMH7, RCM1 |
Research Area | Cancer, Cardiovascular, Developmental biology, Signal transduction, Stem cells |
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
Sample: Cardiomyocytes |
PMID: 32509067 |
Sample: Serum |
PMID: 33185130 |
Sample: Tissue Homogenate |
DOI: 10.1155/2015/283021 |
|
Sample: Serum |
DOI: 10.12659/MSM.919054 |
|
PMID: 31992687 |
Sample: Plasma |
PMID: 35918636 |
Sample: Heart Tissue |
Sample: Serum |
Sample: Serum |
PMID: 35997859 |
Sample: Blood |
Sample: Plasma |
Sample: Serum |
DOI: 10.1002/jcb.26168 |
|
Sample: Serum |
Sample: Serum,Tissue Homogenate |
DOI: 10.3390/life12101466 |
|
PMID: 36294901 |
Sample: Plasma |
PMID: 33159931 |
Sample: Serum |
PMID: 35274595 |
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: Cell Culture Supernatant |
Sample: Bronchoalveolar Lavage Fluid |
DOI: 10.1042/BSR20221306 |
|
PMID: 35959640 |
Sample: Serum |
Sample: Serum |
PMID: 33492555 |
Sample: Cell Culture Supernatant |
PMID: 34322808 |
Sample: Serum |
PMID: 34408544 |
Sample: Serum |
PMID: 35219728 |
Sample: Myocardial |
PMID: 36080137 |
Sample: Serum |
PMID: 34673348 |
Sample: Serum |
Sample: Plasma |
Sample: Serum |
PMID: 36302499 |
Sample: Myocardium,Serum |
DOI: 10.1155/2022/1090893 |
|
Sample: Serum |
Sample: Serum |
PMID: 36195952 |
Sample: Blood |
PMID: 34147720 |
Sample: Serum |