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Ferrous Iron Colorimetric Assay Kit

Cat.No.:E-BC-K773-M
Detection instrument: Microplate reader (590-600 nm, optimum wavelength: 593 nm）

Detection method: Colorimetric method

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Detection principle

Ferrous ions (Fe2+) in samples can bind with probe to form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly.

Performance characteristics

Sample type	serum, tissue
Sensitivity	0.4 μmol/L
Detection range	0.4-50 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	40 min
Precision	Average inter-assay CV: 1.500%Average intra-assay CV: 1.300%
Storage	2-8℃
Valid period	12 months

Images

Ferrous ion level in serum was significantly induced by CCl4 and conversed by SFN. (**P<0.01, ***P<0.001)

J W Liu et al investigate Nrf2 and its dependent autophagy activation cooperatively counteract ferroptosis to alleviate acute liver injury. Ferrous ion level in rat serum were determined using ferrous ion colorimetric assay kit (E-BC-K773-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.4-50 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1-2
Rat serum	1
10% Mouse liver tissue homogenate	1-3
10% Rat lung tissue homogenate	1
10% Mouse heart tissue homogenate	1
10% Rat spleen tissue homogenate	1-3
10% Epipremnum aureum leaf tissue homogenate	1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Small Methods (2021) IF: 14.188
RNA m6A Modification Alteration by Black Phosphorus Quantum Dots Regulates Cell Ferroptosis: Implications for Nanotoxicological Assessment

DOI: 10.1002/smtd.202001045

PMID: 34927824
Sample: Cell sample
EBioMedicine (2022) IF: 11.205
Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis

DOI: 10.1016/j.ebiom.2022.104258

PMID: 36137413
Sample: OA cartilage
PHARMACOLOGICAL RESEARCH (2022) IF: 10.334
Nrf2 and its dependent autophagy activation cooperatively counteract ferroptosis to alleviate acute liver injury

DOI: 10.1016/j.phrs.2022.106563

Sample: serum
JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435
Inhibitory effect and mechanism of gelatin stabilized ferrous sulfide nanoparticles on porcine reproductive and respiratory syndrome virus

DOI: 10.1186/s12951-022-01281-4

PMID: 35123507
Sample: MARC-145 cell
eLife (2023) IF: 8.7129
Neutral amino acid transporter SLC38A2 protects renal medulla from hyperosmolarity-induced ferroptosis

DOI: 10.7554/eLife.80647

Sample: Renal medullary tissue
Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
Mesenchymal Stem Cell-Derived Exosomes Ameliorate Delayed Neurocognitive Recovery in Aged Mice by Inhibiting Hippocampus Ferroptosis via Activating SIRT1/Nrf2/HO-1 Signaling Pathway

DOI: 10.1155/2022/3593294

PMID: 36238648
Sample: hippocampus
Oxidative Medicine and Cellular Longevity (2022) IF: 7.31
Dapagliflozin Ameliorates Renal Tubular Ferroptosis in Diabetes via SLC40A1 Stabilization

DOI: 10.1155/2022/9735555

PMID: 35993021
Sample: Kidney tissue,HK-2 cell
Cells (2022) IF: 7.666
Targeting Wnt/β-Catenin Signaling Exacerbates Ferroptosis and Increases the Efficacy of Melanoma Immunotherapy via the Regulation of MITF

DOI: 10.3390/cells11223580
Frontiers in Oncology (2022) IF: 6.244
Sevoflurane Induces Ferroptosis of Glioma Cells Through Activating the ATF4-CHAC1 Pathway.

DOI: 10.3389/fonc.2022.859621

Sample: U251 cell,U87 cell
Nutrients (2023) IF: 6.706
Lactobacillus johnsonii L531 Ameliorates Salmonella enterica Serovar Typhimurium Diarrhea by Modulating Iron Homeostasis and Oxidative Stress via the IRP2 Pathway

DOI: 10.3390/nu15051127

Sample: IPEC-J2 cell
EXPERIMENTAL NEUROLOGY (2022) IF: 5.62
S100A8 regulates autophagy-dependent ferroptosis in microglia after experimental subarachnoid hemorrhage

DOI: 10.1016/j.expneurol.2022.114171

PMID: 35870523
Sample: Brain tissue,BV2 microglia
Frontiers in Oncology (2022) IF: 5.738
Circular RNA circLMO1 Suppresses Cervical Cancer Growth and Metastasis by Triggering miR-4291/ACSL4-Mediated Ferroptosis

DOI: 10.3389/fonc.2022.858598

Sample: CaSki cell
Frontiers in Pharmacology (2022) IF: 5.811
Tetrandrine Citrate Suppresses Breast Cancer via Depletion of Glutathione Peroxidase 4 and Activation of Nuclear Receptor Coactivator 4-Mediated Ferritinophagy

DOI: 10.3389/fphar.2022.820593

Sample: MCF7 cell,MDA-MB-231 cell
Nutrition & Metabolism (2022) IF: 4.654
Association between iron metabolism and non-alcoholic fatty liver disease: results from the National Health and Nutrition Examination Survey (NHANES 2017–2018) and a controlled animal study

DOI: 10.1186/s12986-022-00715-y

Sample: serum
NEUROSCIENCE LETTERS (2022) IF: 3.197
Dexmedetomidine prevents hemorrhagic brain injury by reducing damage induced by ferroptosis in mice

DOI: 10.1016/j.neulet.2022.136842

PMID: 35995304
Sample: brain
Animals (2022) IF: 3.231
Effect of Zearalenone-Induced Ferroptosis on Mice Spermatogenesis

DOI: 10.3390/ani12213026

Sample: testicular tissue
Bioengineered (2021) IF: 3.269
Downregulation of Circular RNA PSEN1 ameliorates ferroptosis of the high glucose treated retinal pigment epithelial cells via miR-200b-3p/cofilin-2 axis

DOI: 10.1080/21655979.2021.2010369

PMID: 34903141
Sample: ARPE19 cell
Oncology Letters (2022) IF: 2.967
Carboxymethylated pachyman induces ferroptosis in ovarian cancer by suppressing NRF1/HO‑1 signaling

DOI: 10.3892/ol.2022.13281

Sample: SKOV3 cell,Hey cell

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