The Basal Medium Eagle (BME) medium was originally designed by Harry Eagle in 1955 for the culture of Hela cells and mouse fibroblasts. The BME medium is based on BSS, adding 8 vitamins and 13 amino acids. The composition is simple and suitable for various cell lines and special studies. It is one of the most commonly used media in cell culture. In addition, BME medium is also the basis of many other media, such as MEM, DMEM, Glasgow's medium are modified on the basis of BME medium. The BME medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients required for cell culture, but does not contain proteins, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

General Information

Concentration	1×
pH	7.2-7.4
D-Glucose	1000mg/L
HEPES	Negative
L-glutamine	Negative
NaHCO₃	2200mg/L
Phenol red	10mg/L
Storage	2-8℃ ,Shading Light
Shipping	RT
Expiration date	24 months

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Notes

1. This product is for research use only.;
2. This product is sterilized by 0.1μm filtration.;
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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