CAP (Chloramphenicol) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E044

      Size:
      • 96T
      Qty:
      - +
      Price: $112

      sample_type: Tissue,Liver,Honey,Milk,Eggss,Water,Urine,Serum,Feed,Milk powder

      Sensitivity: 0.025 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Chloramphenicol (CAP) in samples, such as, tissue, milk, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, CAP in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-CAP antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of CAP. The concentration of CAP in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicators

      Sensitivity: 0.025 ppb (ng/mL)

      Reaction mode:25, 30 min~ 30 min~15 min.

      Detection limit: Tissue, Liver, Honey, Milk--- 0.0125 ppb; Eggs, Water ---0.05 ppb;

      Urine, Serum, Feed, Milk powder---0.025 ppb.

      Cross-reactivity: Chloramphenicol---100%, Thiamphenicol/Florfenicol0.1%.

      Sample recovery rate: Tissue, Liver--- 85%±20%; Honey --- 85%±25%; Water ---90%±20%;

                                             Milk, Feed, Milk powder, Eggs ---75%±25%; Urine, Serum--70%±20%.


      Kit components


      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1mL each

           (0 ppb, 0.025 ppb, 0.075 ppb, 0.225 ppb, 0.675 ppb, 2.025 ppb)

      HRP Conjugate

      11 mL

      Antibody Working Solution

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      Reconstitution Buffer

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy



      Other materials required but not supplied

      Instrument: Microplate reader, Printer, Homogenizer, Nitrogen Evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision transferpettor: Single channel (20-200μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: Ethyl acetate, N-hexane, Acetonitrile, CH3COONa, Acetic acid, Na2Fe(CN)5(NO)·2H2O, β-glucuronidase (activity1,000,000 units/g), ZnSO4·7H2O.


      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date:  expiration date is on the packing box.

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