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Cat.No.:E-BC-K300-M
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Protocols for metabolism assays
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Synonyms | Cu |
Sample type | Serum,plasma,tissue |
Sensitivity | 1.84 μmol/L |
Detection range | 1.84-60 μmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 30 min |
Precision | Average inter-assay CV: 3.100%Average intra-assay CV: 3% |
Other instruments required | Micropipettor, Incubator |
Storage | 2-8℃ |
Valid period | 12 months |
No significant difference was observed in hippocampus, frontal cortex and thalamus in mice subject to KA when treatment with DPA, compared with vehicle group.
N Yang et al investigate the seizure-induced neurotoxicity revealed by D-penicillamine. Copper (Cu) levels in mouse brain were determined using copper colorimetric assay kit (E-BC-K300-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.84-60 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Human plasma |
1 |
Dog serum |
1 |
Rat serum |
1 |
Rabbit serum |
1 |
Porcine serum |
1 |
Note: The diluent is double distilled water.
Sample: OSRC-2 cells |
PMID: 36009321 |
Sample: brain |
DOI: 10.3390/ijms24021667 |
|
Sample: serum,myocardial tissue |
PMID: 34390123 |
Sample: SW480 cell,DLD-1 cell |
PMID: 33498600 |
Sample: Cell culture medium |
PMID: 34707479 |