Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> FITC Anti-Human CD22 Antibody[BU59] E-AB-F1047C
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|Alternate Names||B-cell receptor CD22,Cd22,B-lymphocyte cell adhesion molecule,BL-CAM,Sialic acid-binding Ig-like lectin 2,Siglec-2,T-cell surface antigen Leu-14,CD22,Lyb-8, Siglec2|
1. Clark E. 1993. J. Immunol. 150:4715.
2. Shan D, et al. 1995. J. Immunol. 154:4466.
|Clone No.||BU59(See Other Available Formats)|
|Isotype||Mouse IgG1, κ|
|Isotype Control||FITC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792C]|
|Application||FCM (See the Application Description)|
|Storage Buffer||Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.|
|Recommended Use||Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.|
|Shipping||Biological ice pack at 4℃|
|Stability & Storage||Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
|FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).|
How to select the appropriate detection channel through the spectrogram?
|CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. It is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.|
|Related Protocols||Staining Cell Surface Targets for Flow Cytometry|
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