Alternate Names	CD99, MIC2, HBA71, MSK5X, E2 antigen
Swissprot	P14209
Gene ID	4267
References	1. Gelin C, et al. 1989. EMBO. 8:3253. 2. Goodfellow PJ, et al. 1986. Science 234:740. 3. Pettersen RD, et al. 2001. J. Immunol. 166:4931.

Product Details

Form	Liquid
Clone No.	HI156(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2a, κ
Isotype Control	FITC Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802C]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	FITC
	FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
PE Anti-Human CD99 Antibody[HI156]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD99 Antibody[HI156]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD99 Antibody[HI156]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD99 Antibody[HI156]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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