Glasgow's MEM (GMEM) was originally developed by Ian McPherson and Michael Stoker based on MEM basal medium to study the ability of genetic factors to affect cells. Glasgow's MEM can also be used for the culture of renal cell lines such as BHK-21. The concentration of amino acids and vitamins in Glasgow's MEM medium was twice that of BME (Basal Medium Eagle) medium. Use of this product requires 10 % tryptone phosphate broth without serum.

General Information

Concentration	1×
pH	7.2-7.4
D-Glucose	4500mg/L
HEPES	Negative
L-glutamine	2mM
NaHCO₃	2750mg/L
Phenol red	15mg/L
Earle’s Balanced Salt	Positive
NEAA	Negative
Storage	2-8℃ ,Shading Light
Shipping	RT
Expiration date	24 months

Notes

1. This product is for research use only._x000D_
2. It is necessary to pay attention to the aseptic operation and avoid the pollution.

Technical Resources

Recommendations for handling of cryopreserved cells

Protocol for Thawing Frozen Cells

Count the number of cells with Trypan Blue exclusion

Citations

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