Click here to view cell apoptosis assay products
×

H+K+-ATPase Activity Assay Kit

  • Cat.No.:E-BC-K122-S

  • Detection instrument: Spectrophotometer(660 nm)

To Purchase E-BC-K122-S

Size:
  • 100Assays
  • 200Assays
Price: $180
Qty:

Detection principle

ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.

Performance characteristics

Sample type Animal tissue,cells
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 60 min
Precision Average inter-assay CV: 9.800000000000001%Average intra-assay CV: 4.4%
Other instruments required Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage Reagent 4: -20℃, others: 2-8℃
Valid period 6 months

Dilution of sample

Dilute the 10% tissue homogenate to different concentrations of 2%, 1%, and 0.5%, then take 100 μL for pre-experiment according to the operation steps. The OD value of sample tube should be less than 1.0.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Animal tissue homogenate

5

GES-1 cells (2.52mg/mL)

1

Note: The diluent is normal saline (0.9% NaCl).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for FREE TRIAL Fluorometric Assay kit
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: