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L-Lactic Acid (LA) Colorimetric Assay Kit

Cat.No.:E-BC-K044-M
Detection instrument: Microplate reader(520-540 nm,optimum wavelength: 530 nm)

Detection method: Colorimetric method

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Detection principle

Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated.

Performance characteristics

Sample type	Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity	0.10 mmol/L
Detection range	0.12-7.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 3.500%Average intra-assay CV: 1.400%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

Lactate secretion was significantly increased in HG group. (*P<0.05)

H Mu at al investigate the glucose metabolism that controlled human tumor immunosurveillance in diabetes. L-Lactate (L-LA) of cell supernatant was determined using L-lactate colorimetric assay kit (E-BC-K044-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-7.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	2-5
10% Rat kidney tissue homogenate	1-3
10% Rat brain tissue homogenate	1
HepG2 cell culture supernatant	1
HepG2 cells	1

Note: The diluent is normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

CELL (2023) IF: 66.849
Light modulates glucose metabolism by a retina-hypothalamus-brown adipose tissue axis

DOI: 10.1016/j.cell.2022.12.024

PMID: 36669474
Sample: Serum
Cellular & Molecular Immunology (2022) IF: 22.096
Glucose metabolism controls human γδ T-cell-mediated tumor immunosurveillance in diabetes

DOI: 10.1038/s41423-022-00894-x

PMID: 35821253
Sample: serum
Theranostics (2021) IF: 11.556
Activation of Hippo signaling pathway mediates mitochondria dysfunction and dilated cardiomyopathy in mice

DOI: 10.7150/thno.62302

PMID: 34522223
Sample: heart tissue
Journal of Neuroinflammation (2022) IF: 8.3219
Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer’s disease-like mice

DOI: 10.1186/s12974-022-02521-y

Sample: microglia
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2023) IF: 7.088
PKM2/PDK1 dual-targeted shikonin derivatives restore the sensitivity of EGFR-mutated NSCLC cells to gefitinib by remodeling glucose metabolism

DOI: 10.1016/j.ejmech.2023.115166

Sample: H1975 cell
Genes & Diseases (2022) IF: 7.243
Integrated analysis reveals the protective mechanism and therapeutic potential of hyperbaric oxygen against pulmonary fibrosis

DOI: 10.1016/j.gendis.2022.08.012

Sample: lung tissue
Cell Communication and Signaling (2022) IF: 7.525
Inhibition of AMPK/PFKFB3 mediated glycolysis synergizes with penfluridol to suppress gallbladder cancer growth

DOI: 10.1186/s12964-022-00882-8

PMID: 35842652
Sample: SGC-996 cell,EH-GB1 cell,GBC-SD cell
Cancer Cell International (2023) IF: 6.429
Coordinated reprogramming of renal cancer transcriptome, metabolome and secretome associates with immune tumor infiltration

DOI: 10.1186/s12935-022-02845-y

Sample: KIJ265T cell,KIJ308T cell,RPTEC/TERT1 cell,Caki-1
CANCER SCIENCE (2022) IF: 6.518
circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

DOI: 10.1111/cas.15351

Sample: AGS cell,HGC-27 cell
iScience (2021) IF: 5.458
Excipient of paclitaxel induces metabolic dysregulation and unfolded protein response

DOI: 10.1016/j.isci.2021.103170

PMID: 34646996
Sample: THP-1 cell
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Canagliflozin Modulates Hypoxia-Induced Metastasis, Angiogenesis and Glycolysis by Decreasing HIF-1α Protein Synthesis via AKT/mTOR Pathway

DOI: 10.3390/ijms222413336

Sample: HepG2 cell
Journal of Cancer (2021) IF: 4.207
18F-FDG PET/CT metabolic parameters correlate with EIF2S2 expression status in colorectal cancer

DOI: 10.7150/jca.57926

Sample: SW480 cell
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2023) IF: 3.094
Metabolic Phenotype Intricacies on Altered Glucose Metabolism of Breast Cancer Cells upon Glut-1 Inhibition and Mimic Hypoxia In Vitro

DOI: 10.1007/s12010-023-04373-5

Sample: SKBR-3 breast cancer cell
REPRODUCTION (2020) IF: 3.206
Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats

DOI: 10.1530/REP-20-0381

PMID: 33112813
Sample: testis
ACS Omega (2022) IF: 3.512
Specific Tumor Cell Detection by a Metabolically Targeted Aggregation-Induced Emission-Based Gold Nanoprobe

DOI: 10.1021/acsomega.2c01494

Sample: MCF-7 cell,HepG2 cell,Caco-2 cell
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK (2022) IF: 3.821
Knockdown of circGOLPH3 inhibits cell progression and glycolysis by targeting miR-145-5p/lysine demethylase 2A (KDM2A) axis in oral squamous cell carcinoma

DOI: 10.1002/hed.27229

PMID: 36268878
Sample: CAL27 cells,HSC3 cells
Biomedical Engineering Online (2021) IF: 2.819
Ribonucleic acid-binding protein CPSF6 promotes glycolysis and suppresses apoptosis in hepatocellular carcinoma cells by inhibiting the BTG2 expression

DOI: 10.1186/s12938-021-00903-6

PMID: 34217312
Sample: Huh-8 cells
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES (2022) IF: 2.945
CircCDC6 restrains tumor growth and glycolysis energy metabolism in colorectal cancer via regulating miR-3187-3p and downstream PRKAA2

DOI: 10.1007/s10863-022-09938-3

PMID: 35438362
Sample: SW480 cell,HCT116 cell

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