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Cat.No.:E-BC-K013-M
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Synonyms | NEFA |
Sample type | Animal tissue |
Sensitivity | 0.15 mmol/L |
Detection range | 0.15-1.5 mmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 30 min |
Precision | Average inter-assay CV: 5.100%Average intra-assay CV: 3.300% |
Other instruments required | Vortex mixer, Micropipettor, Multichannel transferpettor (300 μL) |
Storage | 2-8℃ |
Valid period | 12 months |
Intracellular free fatty acid was significantly reduced in shM5-1 and shM5-2 group. (P<0.0001)
H Peng et al investigate the effect m6A in 18S rRNA of promoting fatty acid metabolism and oncogenic transformation. Non-esterified Free Fatty Acid in Human cell was determined using NEFA colorimetric assay kit (E-BC-K013-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.15-1.5 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Rat liver tissue homogenate |
1 |
Rat heart tissue homogenate |
1 |
Rat kidney tissue homogenate |
1 |
Mouse liver tissue homogenate |
1 |
Note: The diluent is Reagent 1.
Sample: B16-F10 cell,CT-26 cell |
PMID: 36089014 |
Sample: liver organoids (LOs) |
PMID: 34571217 |
Sample: liver organoids (LOs) |
DOI: 10.1111/pai.13651 |
|
Sample: Beas-2b cell |
DOI: 10.3390/nu14030440 |
|
PMID: 35276798 |
Sample: plasma |
Sample: Tissue homogenate |
DOI: 10.2147/CLEP.S313343 |
|
PMID: 34135637 |
Sample: blood |
Sample: Serum |
PMID: 33033766 |
Sample: Plasma,Tissue homogenate |
DOI: 10.5650/jos.ess21389 |
|
PMID: 35491096 |
Sample: serum |
M*****iSubmitted [ Oct 27 2022 ]
Asked: Is it required to use fresh sample?
Reply
adminSubmitted [ Oct 27 2022 ]
Answered: Yes, the samples should be fresh collected and detect within 24 hours.
M*****iSubmitted [ Sep 23 2022 ]
Asked: Can this kit be used to measure the non-esterified free fatty acids (NEFA) content in rat serum sample?
Reply
adminSubmitted [ Sep 23 2022 ]
Answered: Unfortunately, this kit is not suitable for serum sample.You can choose E-BC-K013-S to detect rat serum sample.