Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine5 Anti-Mouse/Rat Foxp3 Antibody[FJK-16s]
Cat.No.:E-AB-F1351UG
Welcome to order from local distributors.
FAQ for Flow Cytometry Antibody
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Alternate Names | Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2 |
Swissprot | Q99JB6 |
Gene ID | 20371 |
References |
1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057. 3. Fontenot JD, et al. 2003 Nature Immunol 4:330. |
Form | Liquid |
Clone No. | FJK-16s(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | PE/Cyanine5 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833G] |
Reactivity | Mouse, Rat |
Application | ICFCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE/Cyanine5 |
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
Foxp3 is a 47 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of Foxp3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. Foxp3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of Foxp3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. |
Related Protocols | Staining Intracellular Antigens for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
FITC Anti-Mouse/Rat Foxp3 Antibody[FJK-16s] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Mouse/Rat Foxp3 Antibody[FJK-16s] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse/Rat Foxp3 Antibody[FJK-16s] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5 Anti-Mouse/Rat Foxp3 Antibody[FJK-16s] | 50Tests, 100Tests, 100Tests×2 | Check More |
PerCP/Cyanine5.5 Anti-Mouse/Rat Foxp3 Antibody[FJK-16s] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE/Elab Fluor® 594 Anti-Mouse/Rat Foxp3 Antibody[FJK-16s] | 50Tests, 100Tests, 100Tests×2 | Check More |
FITC Anti-Mouse/Rat Foxp3 Antibody[FJK-16s] | 25μg, 100μg | Check More |
PE Anti-Mouse/Rat Foxp3 Antibody[FJK-16s] | 25μg, 100μg | Check More |
APC Anti-Mouse/Rat Foxp3 Antibody[FJK-16s] | 25μg, 100μg | Check More |
PerCP/Cyanine5.5 Anti-Mouse/Rat Foxp3 Antibody[FJK-16s] | 25μg, 100μg | Check More |
PE/Elab Fluor® 594 Anti-Mouse/Rat Foxp3 Antibody[FJK-16s] | 25μg, 100μg | Check More |