Carbohydrate is one of the important components of plant corpus, and is also the main raw material of metabolism and storage material. The kit is used for the determination of soluble monosaccharides, oligosaccharides and polysaccharides. It has the advantages of high sensitivity, quick convenience and suitable for the determination of microscale samples. The detection principle is anthrone colorimetry. Carbohydrate react with anthrone to produce colored material, which has the maximum absorption peak at 620 nm. The soluble sugar content can be determined by measuring the absorbance value.

Performance characteristics

Sample type	plant sample
Detection range	0.003-1.5 mg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 5.300%Average intra-assay CV: 1.700%
Other instruments required	Centrifuge
Other reagents required	Concentrated sulfuric acid, Ethyl acetate
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

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Cat. No	E-BC-K866-M
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