Current Position:Home>>Kits>>ELISA Kits>> Porcine ACTH(Adrenocorticotropic Hormone) ELISA Kit
Cat.No.:E-EL-P0185
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Assay type | Competitive-ELISA |
Format | 48T/96T/96 |
Assay time | 2.5h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample type &Sample volume | ; 50μL |
Specificity | This kit recognizes ACTH in samples. No significant cross-reactivity or interference between ACTH and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of ACTH concentrations in . |
Item | Specifications | Storage |
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Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ACTH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ACTH were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 50.87 | 137.14 | 399.81 | 52.45 | 128.28 | 373.56 |
Standard deviation | 2.66 | 5.69 | 21.55 | 3.66 | 7.13 | 18.53 |
CV (%) | 5.23 | 4.15 | 5.39 | 6.98 | 5.56 | 4.96 |
The recovery of ACTH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 90-104 | 96 |
EDTA plasma(n=8) | 92-106 | 99 |
Cell culture media(n=8) | 91-103 | 96 |
Samples were spiked with high concentrations of Porcine ACTH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt: P01192(136-174aa) |
Synonyms | Corticotropin;POMC;Adrenocorticotropin |
Research Area | Cancer, Metabolism, Neuroscience, Signal transduction |
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C |
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2. Aspirate and wash the plate for 3 times |
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3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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5. Add 50μL Stop Solution |
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6. Read the plate at 450nm immediately. Calculation of the results |