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Cat.No.:E-AB-F1212A
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Alternate Names | Bp35,Leukocyte surface antigen Leu-16,MS4A1,B-lymphocyte surface antigen B1 |
Swissprot | P11836 |
References |
1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450. |
Form | Liquid |
Clone No. | 2H7(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG2b, κ |
Isotype Control | Purified Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F09813A] |
Reactivity | Human |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Unconjugated |
How to select the appropriate detection channel through the spectrogram?
CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
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PE Anti-Human CD20 Antibody[2H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
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PE/Cyanine5 Anti-Human CD20 Antibody[2H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
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PE/Cyanine5.5 Anti-Human CD20 Antibody[2H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
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Elab Fluor® 488 Anti-Human CD20 Antibody[2H7] | 20Tests, 100Tests, 100Tests×2 | Check More |
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