Rat Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit

    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
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    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience

      Catalog number:E-EL-R1430

      Synonyms:Angiotensin-2

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Rat

      Detection Range: 15.63~1000 pg/mL

      Sensitivity: 9.38 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Rat Ang-Ⅱ concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Ang-Ⅱ. During the reaction, Rat Ang-Ⅱ in the sample or standard competes with a fixed amount of Rat Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Ang-Ⅱ in the samples is then determined by comparing the OD of the samples to the standard curve.

      Assay type Competitive
      Format 96T
      Assay time 2.0h
      Reactivity Rat
      Detection Method Colormetric
      Detection Range 15.63—1000 pg/mL
      Sensitivity 9.38 pg/mL
      Sample Volume 50μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Rat Ang-Ⅱ in samples. No significant cross-reactivity or interference between Rat Ang-Ⅱ and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration(pg/mL) O.D Average
      1000 0.358
      0.366
      0.362
      500 0.444
      0.502
      0.473
      250 0.683
      0.655
      0.669
      125 0.961
      0.993
      0.977
      62.5 1.386
      1.364
      1.375
      31.25 1.792
      1.772
      1.782
      15.63 2.101
      2.115
      2.108
      0 2.57
      2.578
      2.574

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat Ang-Ⅱ were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat Ang-Ⅱ were tested on 3 different plates, 20 replicates in each plate.

        Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 46.80 133.70 360.10 46.10 133.40 382.00
      Standard deviation 3.00 7.60 13.00 3.20 7.10 15.70
      C V (%) 6.41 5.68 3.61 6.94 5.32 4.11

      Recovery

      The recovery of Rat Ang-Ⅱ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 88-100 94
      EDTA plasma (n=5) 90-104 96
      Cell culture media (n=5) 84-96 91

      Linearity

      Samples were spiked with high concentrations of Rat Ang-Ⅱ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

          Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 85-97 92-107 94-107
      Average (%) 90 98 100
      1:4 Range (%) 84-95 91-103 96-108
      Average (%) 90 97 102
      1:8 Range (%) 86-97 90-102 98-113
      Average (%) 92 96 104
      1:16 Range (%) 89-100 87-101 93-108
      Average (%) 94 94 100

      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 4℃(shading light)
      Stop Solution 1 vial, 10 mL 4℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      1.Add 50 μL standard or sample to each well.

      2.Immediately add 50 μL Biotinylated Detection Ab to each well.

      3.Incubate for 45 min at 37℃. Aspirate and wash 3 times.

      4.Add 100 μL HRP Conjugate to each well. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      5.Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃.

      6.Add 50 μL Stop Solution.

      7.Read at 450 nm immediately. Calculation of results.

      Citations

      1. Publication: Zhou Q, Pan X, Wang L, et al. The Protective Role Of Neuregulin-1: A Potential Therapy For Sepsis-Induced Cardiomyopathy[J]. European Journal of Pharmacology, 2016, 788: 234-240.
        Sample Type: Serum
      2. Publication: Hohl M. Modulation Of The Sympathetic Nervous System By Renal Denervation Prevents Reduction Of Aortic Distensibility In Atherosclerosis Prone ApoE-Deficient Rats[J]. Journal of Translational Medicine, 2016.
        Sample Type: Tissue homogenates
      3. Publication: Yuan P P, Zheng X K, Li M, et al. Two Sulfur Glycoside Compounds Isolated From Lepidium Apetalum Willd Protect NRK52e Cells Against Hypertonic-Induced Adhesion And Inflammation By Suppressing The MAPK Signaling Pathway And RAAS[J]. Molecules, 2017.
        Sample Type: culture supernatant
      4. Publication: Amara V R, Surapaneni S K, Tikoo K. Dysregulation Of MicroRNAs And Renin-Angiotensin System In High Salt Diet-Induced Cardiac Dysfunction In Uninephrectomized Rats[J]. PloS one, 2017.
        Sample Type: Plasma
      5. Publication: Zeng M, Zhang L, Li M, et al. Mechanism of the diuretic activity of Descurainia sophia seed[J]. Bangladesh Journal of Pharmacology, 13(2): 157-163, May 15, 2018.
        Sample Type: Rat
      6. Publication: Liu J, Guo L, Zhang K, et al. The probable roles of valsartan in alleviating chronic obstructive pulmonary disease following co-exposure to cold stress and fine particulate matter[J]. Environmental Toxicology and Pharmacology, 2018, 60:230.
        Sample Type: Rat
      7. Publication: K. Zhang et al. COPD rat model is more susceptible to cold stress and PM2.5 exposure and the underlying mechanism [J]. Environmental Pollution 241 (2018) 26-34.
        Sample Type: Rat
      8. Publication: Yuan P , Zheng X , Li M , et al. Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS[J]. Molecules, 2017, 22(11):1956.
        Sample Type: Rat
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