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Rat IGF-1(Insulin-like Growth Factor 1) ELISA Kit

Cat.No.:E-EL-R3001
Reactivity: Rat

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	3.13-200 ng/mL
Sensitivity	1.88 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat IGF-1 in samples. No significant cross-reactivity or interference between Rat IGF-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat IGF-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IGF-1. You can calculate the concentration of Rat IGF-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IGF-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IGF-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	9.11	22.37	91.33	8.97	23.14	83.30
Standard deviation	0.54	1.09	4.33	0.47	1.08	4.10
CV (%)	5.93	4.87	4.74	5.24	4.67	4.92

Recovery

The recovery of Rat IGF-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	97-108	103
EDTA plasma (n=8)	96-109	102
Cell culture media (n=8)	87-97	94

Linearity

Samples were spiked with high concentrations of Rat IGF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	IGF1, IGFI, IGF-I, IGF1A, IGF-IA, IGF-IB, MGF, Somatomedin C
Research Area	Cancer, Developmental biology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Materials Today Advances (2023) IF: 9.9179

Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury

DOI: 10.1016/j.mtadv.2023.100349
	Sample: bone marrow mesenchymal stem cells (BMSCs)

Biomedicines (2022) IF: 6.081

The Role of Neuregulin-1 in Steatotic and Non-Steatotic Liver Transplantation from Brain-Dead Donors

DOI: 10.3390/biomedicines10050978
PMID: 35625715	Sample: liver tissue

Frontiers in Nutrition (2022) IF: 6.576

Effects and Reversibility of Pre- and Post-natal Iron and Omega-3 Fatty Acid Deficiency, Alone and in Combination, on Bone Development in Rats

DOI: 10.3389/fnut.2021.802609
	Sample: plasma

LIFE SCIENCES (2022) IF: 5.037

Studies on testicular ultrastructural and hormonal changes in type-2 diabetic rats treated with highly active antiretroviral therapy conjugated silver nanoparticles

DOI: 10.1016/j.lfs.2022.120498
PMID: 35341824	Sample: testes

BRAIN RESEARCH BULLETIN (2021) IF: 4.079

Role of protein tyrosine phosphatase 1B inhibitor in central insulin resistance and associated cognitive deficits

DOI: 10.1016/j.brainresbull.2021.02.026
PMID: 33684458	Sample: Tissue homogenate

Scientific Reports (2021) IF: 4.38

A nano phototheranostic approach of toluidine blue conjugated gold silver core shells mediated photodynamic therapy to treat diabetic foot ulcer

DOI: 10.1038/s41598-021-04008-x
PMID: 34961769	Sample: serum

EXPERIMENTAL GERONTOLOGY (2019) IF: 3.08

Pharmacological investigation of ‘HIM-CHX’: A herbal combination in the experimental muscle wasting condition

DOI: 10.1016/j.exger.2019.110663
	Sample: Serum

GENERAL AND COMPARATIVE ENDOCRINOLOGY (2022) IF: 3.255

The effect of genistein on IGF-1, PlGF, sFLT-1 and fetoplacental development

DOI: 10.1016/j.ygcen.2022.114122
PMID: 36063867	Sample: placental,serum

PEDIATRIC RESEARCH (2020) IF: 3.756

Human ucMSCs seeded in a decellularized kidney scaffold attenuate renal fibrosis by reducing epithelial–mesenchymal transition via the TGF-β/Smad signaling pathway

DOI: 10.1038/s41390-019-0736-6
PMID: 31896126	Sample: Cell lysate

Bosnian Journal of Basic Medical Sciences (2022) IF: 3.759

Tenofovir-silver nanoparticles conjugate ameliorates neurocognitive disorders and protects ultrastructural and cytoarchitectonic properties of the prefrontal cortex in diabetic rats

DOI: 10.17305/bjbms.2021.6699
	Sample: prefrontal cortex (PFC)

GROWTH HORMONE & IGF RESEARCH (2022) IF: 2.372

Effects of high-dose all-trans retinoic acid on longitudinal bone growth of young rats

DOI: 10.1016/j.ghir.2022.101446
PMID: 35149382	Sample: serum

Frontiers in Surgery (2021) IF: 2.718

The Effect of Waterpipe Tobacco Smoking on Bone Healing Following Femoral Fractures in Male Rats

DOI: 10.3389/fsurg.2021.722446
	Sample: serum

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2022) IF: 2.723

Impact of repeated intravenous infusions of umbilical cord–derived versus bone marrow–derived mesenchymal stem cells on angiogenesis in a pregnant experimentally induced deep venous thrombosis rat model

DOI: 10.1007/s11626-022-00733-3
	Sample: serum

JOURNAL OF INVESTIGATIVE SURGERY (2018) IF: 1.122

Research on the Effects of Levetiracetam in Spinal Cord Injury Model in Rats: An Experimental Study

DOI: 10.1080/08941939.2018.1490828
PMID: 30204031	Sample: Tissue homogenate

Annals of Transplantation (2021) IF: 1.53

Amniotic Fluid Transplantation Alleviates Hematopoietic Deficits in Experimental Rat Aplastic Anemia

DOI: 10.12659/AOT.928047
PMID: 33414361	Sample: The amniotic fluid samples

YONSEI MEDICAL JOURNAL (2018) IF: 1.564

Effect of the Orally Active Growth Hormone Secretagogue MK-677 on Somatic Growth in Rats

DOI: 10.3349/ymj.2018.59.10.1174
PMID: 30450851	Sample: Serum,Tissue homogenate

International Journal of Clinical and Experimental Pathology (2021)

Involvement of IGF/IGFBP/Erk axis in the exercise-mediated preventive effects on colorectal cancer in rats


PMID: 34093946	Sample: Serum

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