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Recombinant HCV (HCV-1a) NS3 protease / helicase immunodominant region Protein (aa 1356-1459, GST Tag)

  • Cat.No.:PKSV030179

  • Expression host: E.coli

To Purchase PKSV030179

Size:
  • 100μg
Price: $855
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Description

Synonyms NS3 Protein;HCV
Species HCV
Expression_host E.coli
Sequence Thr1356-Thr1459
Accession NP_803144.1
Mol_Mass 37.9 kDa
Tag N-GST

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level Please contact us for more information.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 50 mM Tris, 500 mM NaCl.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

HCV NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. HCV NS3 serine protease, in association with NS4A, is responsible for the cleavages of NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS3/NS4A complex also prevents phosphorylation of human IRF3, thus preventing the establishment of dsRNA induced antiviral state. HCV NS3 RNA helicase binds to RNA and unwinds dsRNA in the 3' to 5' direction, and likely RNA stable secondary structure in the template strand (By similarity). Cleaves and inhibits the host antiviral protein MAVS.

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