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Recombinant Human Apolipoprotein A-I/ApoAI Protein (His Tag, E. coli)

Uniprot : P02647

Cat.No.:PKSH032081
Expression host: E.coli

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Description

Synonyms	Apolipoprotein A-I;Apo-AI;ApoA-I;Apolipoprotein A1;APOA1
Species	Human
Expression_host	E.coli
Sequence	Arg19-Gln267
Accession	P02647
Mol_Mass	30.2 kDa
AP_Mol_Mass	28 kDa
Tag	C-His

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 6% Sucrose, 4% Mannitol, 50mM Nacl, 0.05% Tween 80, pH7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specifi
Reconstitution	Please refer to the printed manual for detailed information.

Background

Apolipoprotein A1 (APOA1) is a secreted protein which belongs to the Apolipoprotein A1/A4/E family. APOA1 is the major protein component of high density lipoprotein (HDL) in plasma. APOA1 plays a critical role in various biological processes; such as Cholesterol metabolism; Lipid metabolism and transport; Steroid metabolism. APOA1 promotes cholesterol efflux from tissues to the liver and thus helps to clear cholesterol from arteries. Defects in this gene resulted in HDL deficiencies; including Tangier disease (TGD); systemic non-neuropathic amyloidosis; premature coronary artery disease; hepatosplenomegaly and progressive muscle wasting and weakness. In addition; ApoA-I is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin.

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