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Recombinant Human Carbonic Anhydrase 10/CA10 Protein (Human Cells, His Tag)

Uniprot : Q9NS85

Cat.No.:PKSH032157
Expression host: HEK293 Cells

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Description

Synonyms	Carbonic Anhydrase-Related Protein 10;Carbonic Anhydrase-Related Protein X;CA-RP X;CARP X;Cerebral Protein 15;CA10;CA-RPX;CARPX;HUCEP-15
Species	Human
Expression_host	HEK293 Cells
Sequence	Gln22-Asn300
Accession	Q9NS85
Mol_Mass	32.8 kDa
AP_Mol_Mass	34 kDa
Tag	C-His
Bio_Activity	Not validated for activity

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Carbonic Anhydrase X (CA10) belongs to CA family of zinc metalloenzymes; which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration; renal tubular acidification and bone resorption. While CA10 is a secreted protein without Carbonic Anhydrase activity (i.e.; the reversible hydration of CO2) due to point mutations in the zinc binding site; it has esterase activity. The human and mouse CA10 are expressed in the brain; indicating that they may play a role in brain development.

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