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Recombinant Human Carbonic Anhydrase 8/CA8 Protein (His Tag)

Uniprot : P35219

Cat.No.:PKSH031586
Expression host: E.coli

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100μg
1mg

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Description

Synonyms	Carbonic Anhydrase-Related Protein;CARP;Carbonic Anhydrase VIII;CA-VIII;CA8;CALS;CAMRQ3;MGC120502;MGC99509
Species	Human
Expression_host	E.coli
Sequence	Met 1-Gln 290
Accession	NP_004047.3
Mol_Mass	33.8 kDa
AP_Mol_Mass	37 kDa
Tag	C-His
Bio_Activity	Measured by its esterase activity. The specific activity is > 100 pmoles/min/μg.

Properties

Purity	> 94 % as determined by reducing SDS-PAGE.
Endotoxin level	Please contact us for more information.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, 15% glycerol, pH 7.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.

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