Synonyms	B-Lymphocyte Antigen CD19;B-Lymphocyte Surface Antigen B4;Differentiation Antigen CD19;T-Cell Surface Antigen Leu-12;CD19;CVID3
Species	Human
Expression_host	CHO Stable Cells
Sequence	Pro20-Lys291
Accession	P15391
Application	Functional ELISA
Mol_Mass	57.3 kDa
AP_Mol_Mass	80-95 kDa
Tag	C-Fc
Bio_Activity	Immobilized Human FMC63 at 2μg/ml(100 μl/well) can bind Human CD19-Fc. The ED₅₀ of Human CD19-Fc is 55.28 ug/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed man
Reconstitution	Please refer to the printed manual for detailed information.

Background

CD19 is a single-pass type I membrane protein containing 2 Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact; it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. CD19 primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation; the cytoplasmic tail of CD19 becomes phosphorylated; which leads to binding by Src-family kinases and recruitment of PI-3 kinase. CD19 Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) which is a primary immunodeficiency characterized by antibody deficiency; hypogammaglobulinemia; recurrent bacterial infections and an inability to mount an antibody response to antigen.

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